PE anti-CD247 (TCRζ, CD3ζ) Antibody

Pricing & Availability
Clone
6B10.2 (See other available formats)
Other Names
CD247, CD3zeta
Isotype
Mouse IgG1 κ
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Product Citations
publications
6B10dot2_PE_CD247_Antibody_ICFC_1_052115
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with Fixation Buffer and the Permeabilization buffer. Cells were then stained with CD247 (clone 6B10.2) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 6B10dot2_PE_CD247_Antibody_ICFC_1_052115
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with Fixation Buffer and the Permeabilization buffer. Cells were then stained with CD247 (clone 6B10.2) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 6B10dot2_PE_CD247_Antibody_ICFC_2_052115
See PE spectral data
Cat # Size Price Quantity Avail. Save
644105 25 µg $125
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644106 100 µg $295
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Description

The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TCR zeta chain aa38-54
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.2 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhang Z, et al. 2007. Blood 109:4328. (Block)
  2. Gorman CL, et al. 2008. J. Immunol. 180:1060.
  3. Jenson WA, et al. 1997. Eur J. Immunol. 27:707.
  4. Mao H, et al. 2010. J. Virol. 84:4148. PubMed
  5. Hwang I, et al. 2012. Int Immunol. 24:793. PubMed.
RRID
AB_2565720 (BioLegend Cat. No. 644105)
AB_2565721 (BioLegend Cat. No. 644106)

Antigen Details

Structure
163 amino acid long protein with a short extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution
T cells and NKT cells. Reported also positive on CD16+ human NK cells.
Function
Mediating signal transduction after TCR receptor engagement.
Interaction
ZAP70, TCR alpha/beta
Cell Type
T cells, NKT cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
TCRs, CD Molecules
Antigen References

1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709

Gene ID
919 View all products for this Gene ID
UniProt
View information about CD247 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05/21/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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