Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Other Names
Sca-1
Isotype
Rat IgG2a, κ
Ave. Rating
2 reviews
Product Citations
publications
D7_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ D7
  • D7_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ D7
See Pacific Blue™ spectral data
Cat # Size Price Quantity Avail. Save
108119 25 µg $85
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108120 100 µg $195
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .
Concentration
0.5 mg/ml
Storage & Handling
The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Agarwal A, et al. 2008. Blood. 112:1960. PubMed
  2. Grassinger J, et al. 2009. Blood. 114:49:00. PubMed
  3. Nemeth M, et al. 2009. Stem Cells. 27:1109. PubMed
  4. Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed
  5. Aguilo F, et al. 2011. Blood. 117:5057. PubMed
  6. Torreggiani E, et al. 2013. PLoS One. 8:75204. PubMed
  7. Ambrosio F, et al. 2014. Free Radic Biol Med. 74:64. PubMed
  8. Karamitros D, et al. 2015. Development. 142:70. PubMed
  9. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  10. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  11. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  12. Quarta M, et al. 2016. Nat Biotechnol. 10.1038/nbt.3576. PubMed
  13. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
  14. Dusseault J, et al. 2016. Diabetes. 65: 2652 - 2666. PubMed
  15. Mueller A, et al. 2016. Nature. 540:276-279. PubMed
  16. Gao B, et al. 2018. Aging Cell. 3. PubMed
  17. Vasamsetti SB 2018. Immunity. 49:93. PubMed
  18. Liu L 2018. Cell stem cell. 23:544. PubMed
  19. Sarshad AA 2018. Molecular cell. 71:1040. PubMed
  20. Wu X 2018. Cell. 172:423. PubMed
  21. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  22. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  23. Huang Z, et al. 2017. Cell Metab. 26:493. PubMed
  24. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  25. Xiong X, et al. 2018. Cell Rep. 25:3006. PubMed
  26. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  27. Wosczyna MN, et al. 2019. Cell Rep. 27:2029. PubMed
  28. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
Publication Library
RRID
AB_493274 (BioLegend Cat. No. 108119)
AB_493273 (BioLegend Cat. No. 108120)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
Hematopoietic stem and progenitors, T cells, B cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells, Inhibitory Molecules
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

Related FAQs

There are no FAQs for this product.
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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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