Pacific Blue™ anti-human CD66b Antibody

Pricing & Availability
G10F5 (See other available formats)
Regulatory Status
Other Names
Mouse IgM, κ
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Product Citations
Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Pacific Blue™ (filled histogram) or mouse IgM, κ Pacific Blue™ isotype control (open histogram).
  • G10F5_PB_CD66b_Antibody_FC_032014
    Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Pacific Blue™ (filled histogram) or mouse IgM, κ Pacific Blue™ isotype control (open histogram).
Compare all formats See Pacific Blue™ spectral data
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305111 25 tests $136
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305112 100 tests $335
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CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Reported Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gilchuk P, et al. 2020. Immunity. 52:388. PubMed
  2. Barrett JR, et al. 2021. Nat Med. 27:279. PubMed
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  4. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  5. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  6. Gorman MJ, et al. 2021. Cell Rep Med. 2:100405. PubMed
  7. Jundi B, et al. 2021. JCI Insight. 6:e148866. PubMed
  8. Williamson LE, et al. 2021. Cell. 184:4430. PubMed
  9. Gunn BM, et al. 2021. Immunity. 54(4):815-828.e5. PubMed
  10. House RV, et al. 2022. PLoS One. 17:e0267796. PubMed
  11. Herman JD, et al. 2021. Nat Commun. 12:6853. PubMed
  12. Gunn BM et al. 2018. Cell host & microbe. 24(2):221-233 . PubMed
  13. Chen RE, et al. 2021. Med (N Y). 2:1327. PubMed
  14. Das J, et al. 2021. Med (N Y). 2:1269. PubMed
  15. Offersen R, et al. 2020. Cell Rep. 33:108502. PubMed
  16. Cadena AM, et al. 2021. Nat Commun. 12:1474. PubMed
  17. Alter G, et al. 2020. Cell. 183(1):185-196.e14. PubMed
  18. Minassian AM, et al. 2021. Med (N Y). 2:701. PubMed
  19. Lu LL et al. 2016. Cell. 167(2):433-443 . PubMed
  20. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  21. Dolatshahi S, et al. 2022. Sci Rep. 12:14937. PubMed
  22. Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed
  23. Krampert L, et al. 2021. Front Immunol. 12:712948. PubMed
  24. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  25. Zelter T, et al. 2022. EMBO Rep. 23:e53641. PubMed
  26. Pullen KM, et al. 2021. Cell Rep. 37:109959. PubMed
  27. Bartsch YC, et al. 2021. Nat Commun. 12:1018. PubMed
  28. Jennewein MF, et al. 2020. Cell. 178(1):202-215.e14.. PubMed
  29. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  30. LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed
  31. Om K, et al. 2020. PLoS Pathog. 16:e1008764. PubMed
  32. Ilinykh PA, et al. 2018. PLoS Pathog. 14:e1007204. PubMed
  33. Spolski R et al. 2019. Elife. 8 pii: e45501. PubMed
  34. King LB et al. 2018. Cell host & microbe. 23(1):101-109 . PubMed
  35. Atyeo C, et al. 2022. Nat Commun. 13:3571. PubMed
  36. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  37. Boudreau CM, et al. 2022. Cell Rep. 38:110337. PubMed
  38. Atyeo C, et al. 2021. JCI Insight. 6:. PubMed
  39. Kaplonek P, et al. 2022. Immunity. 55:355. PubMed
  40. Bornholdt ZA et al. 2019. Cell host & microbe. 25(1):49-58 . PubMed
  41. Malherbe DC, et al. 2022. NPJ Vaccines. 7:83. PubMed
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AB_2563293 (BioLegend Cat. No. 305111)
AB_2563294 (BioLegend Cat. No. 305112)

Antigen Details

Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD


Cell adhesion, neutrophil activation
Cell Type
Granulocytes, Neutrophils
Biology Area
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
View information about CD66b on

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Go To Top Version: 3    Revision Date: 01/05/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Alexa Fluor® 647 anti-human CD66b

  • Alexa Fluor® 700 anti-human CD66b

  • PE/Cyanine7 anti-human CD66b

  • APC anti-human CD66b

  • Biotin anti-human CD66b

  • PE/Dazzle™ 594 anti-human CD66b

  • Alexa Fluor® 594 anti-human CD66b

  • APC/Cyanine7 anti-human CD66b

  • FITC anti-human CD66b

  • GMP FITC anti-human CD66b


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