Pacific Blue™ anti-human CD27 Antibody

Pricing & Availability
Clone
M-T271 (See other available formats)
Workshop
V 5T CD27.03
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M-T271_PB_CD27_Antibody_FC_082913
Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) Pacific Blue™ (filled histogram) or mouse IgG1 Pacific Blue™ isotype control (open histogram).
  • M-T271_PB_CD27_Antibody_FC_082913
    Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) Pacific Blue™ (filled histogram) or mouse IgG1 Pacific Blue™ isotype control (open histogram).
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Cat # Size Price Quantity Avail. Save
356413 25 tests $125
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356414 100 tests $285
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reacitivity: Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human T cells from a T-ALL patient.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.

Application References

(PubMed link indicates BioLegend citation)
  1. Ma S, et al. 2011. J. Virol. 85:165. (IHC)
  2. Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF)
  3. Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)
RRID
AB_2562504 (BioLegend Cat. No. 356413)
AB_2562505 (BioLegend Cat. No. 356414)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution
Medullary thymocytes, T and B cell subsets, NK cells
Interaction
CD27 binds to CD70
Ligand Receptor
CD70
Cell Type
Thymocytes, T cells, B cells, NK cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Hintzen R, et al. 1994. Immunol. Today 15:307.
4. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/06/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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