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Pacific Blue™ anti-human CD27 Antibody

Pricing & Availability
Clone
O323 (See other available formats)
Regulatory Status
RUO
Workshop
IV T-186
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
O323_PB_030510
Human peripheral blood lymphocytes stained with O323 Pacific Blue™
  • O323_PB_030510
    Human peripheral blood lymphocytes stained with O323 Pacific Blue™
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
302821 25 µg $141
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302822 100 µg $293
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue??¤?³ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue??¤?³ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
RRID
AB_2073317 (BioLegend Cat. No. 302821)
AB_2244308 (BioLegend Cat. No. 302822)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Function
T cell costimulation
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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