Pacific Blue™ anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
Ave. Rating
5 reviews
Product Citations
publications
HIB19_Blue_022008
Human peripheral blood lymphocytes stained with HIB19 Pacific Blue™
  • HIB19_Blue_022008
    Human peripheral blood lymphocytes stained with HIB19 Pacific Blue™
See Pacific Blue™ spectral data
Cat # Size Price Quantity Avail. Save
302223 25 µg $105
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302224 100 µg $225
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302232 100 tests $240
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Chimpanzee, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
test size: lot-specific; µg sizes: 0.5 mg/ml
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤ 0.5 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Koning D, et al. 2013. J Immunol. 190:391. PubMed
  2. Santos J, et al. 2013. J Transl Med. 11:18. PubMed
  3. Bartholomaeus P, et al. 2014. J Immunol. 192:2091. PubMed
  4. Steinsbø &, et al. 2014. Nat Commun. 5:4041. PubMed
  5. Tungatt K, et al. 2015. J Immunol. 194:463. PubMed
  6. Boer M, et al. 2015. Clin Vaccine Immunol. 22: 778 - 788. PubMed
  7. Linley A, et al. 2015. Blood. 126: 1902 - 1910. PubMed
  8. Autenrieth S, et al. 2015. Clin Transl Immunology. 4:50. PubMed
  9. Della-Torre E, et al. 2015. Ann Rheum Dis. 74: 2236 - 2243. PubMed
  10. Holland C, et al. 2015. J Immunol. 195: 5827 - 5836. PubMed
  11. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  12. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  13. Gauvin J, et al. 2016. PLoS One. 11: 0155868. PubMed
  14. Chen L, et al. 2016. Ann Rheum Dis. 75: 916 - 923. PubMed
  15. Malarkey C, et al. 2016. PLoS One. 11: 0157157. PubMed
  16. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  17. Dockree T, et al. 2016. Immunol Cell Biol. 95:68-76. PubMed
  18. Drennan S, et al. 2016. Leukemia. 10.1038/leu.2016.356. PubMed
  19. Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
  20. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  21. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  22. Chen YQ et al. 2018. Cell. 173(2):417-429 . PubMed
  23. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  24. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  25. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  26. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  27. Dulberger CL et al. 2017. Immunity. 46(6):1018-1029 . PubMed
  28. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  29. Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
RRID
AB_493652 (BioLegend Cat. No. 302223)
AB_493653 (BioLegend Cat. No. 302224)
AB_2073118 (BioLegend Cat. No. 302232)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/18/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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