Normal Serum Block (Previously Covance catalog# SIG-31170)

Pricing & Availability
Other Names
Normal Serum Blocking Solution
Previously
Signet Catalog# 1170
Covance Catalog# SIG-31171
Covance Catalog# SIG-31172
Signet Catalog# 1171
Signet Catalog# 1172
Covance Catalog# SIG-31170
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Product Citations
publications
Normal_Serum_Block_1_HR_090817
Immunofluorescence staining of anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded (FFPE) tissue section from rat cerebellum. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3, the tissue was blocked with 5%NGS + 1%BSA (2nd image) or Normal Serum Block (1st image) for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL (diluted in 5%NGS+1%BSA or Normal Serum Block, respectively) overnight at 4°C. Images were captured with a 40X objective (Scale Bar: 20 µm).
  • Normal_Serum_Block_1_HR_090817
    Immunofluorescence staining of anti-Neurofilament H (NF-H), Nonphosphorylated antibody (clone SMI 32) on formalin-fixed paraffin-embedded (FFPE) tissue section from rat cerebellum. After antigen retrieval using Retrieval-ALL Antigen Unmasking System 3, the tissue was blocked with 5%NGS + 1%BSA (2nd image) or Normal Serum Block (1st image) for 30 min at room temperature, and then incubated with SMI 32 at 5 µg/mL (diluted in 5%NGS+1%BSA or Normal Serum Block, respectively) overnight at 4°C. Images were captured with a 40X objective (Scale Bar: 20 µm).
  • Normal_Serum_Block_2_HR_090817
Cat # Size Price Quantity Avail. Save
927501 6 mL $32
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927502 15 mL $49
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927503 50 mL $96
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Technical data sheet

Product Details

Host Species
Goat
Preparation
Goat Serum
Storage & Handling
Store between 2°C and 8°C.
Application

IHC

Recommended Usage

The optimal working dilution should be determined for each specific assay condition.

Application Notes

Normal goat serum is used as a blocking solution in immunohistochemistry. It is effective in reducing non-specific binding of proteins. For example, Normal Serum Block prevents the secondary antibody from cross reacting with endogenous immunoglobulins in the tissue. The use of normal serum block before the application of primary antibody also eliminates Fc receptor binding of both the primary and secondary antibody.

The Normal Serum Block (cat#s 927501, 927502 ,  927503) is supplied as a blue-colored solution for easy identification when working with multiple IHC reagents. Internal testing has indicated that this product does not interfere with immunofluorescent signals, nor did it contribute to autofluorescence in microscopy work.

Product Citations
  1. Ghanbari L, et al. 2019. Nat Commun. 10:1500. PubMed

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11/23/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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