MojoSort™ Mouse CD45.1 Selection Kit

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Other Names
CD45
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MojoSort_Mouse_CD451_Selection_Kit_1_101618
A single cell suspension with a mix of approximately 10% SJL (CD45.1+) and 90% C57BL/6 (CD45.2+) splenocytes was prepared for the positive selection of CD45.1+ cells using the MojoSort™ Mouse CD45.1 Selection Kit. Left plot: CD45.1+ cells after isolation. Right plot: Cells before isolation. Cells were stained with anti-mouse CD45.1 (clone A20) APC and CD45.2 (clone 104) PE. Dead cells were excluded by Helix NP™ Blue.
  • MojoSort_Mouse_CD451_Selection_Kit_1_101618
    A single cell suspension with a mix of approximately 10% SJL (CD45.1+) and 90% C57BL/6 (CD45.2+) splenocytes was prepared for the positive selection of CD45.1+ cells using the MojoSort™ Mouse CD45.1 Selection Kit. Left plot: CD45.1+ cells after isolation. Right plot: Cells before isolation. Cells were stained with anti-mouse CD45.1 (clone A20) APC and CD45.2 (clone 104) PE. Dead cells were excluded by Helix NP™ Blue.
  • MojoSort_Mouse_CD451_Selection_Kit_2_101618
    A single cell suspension with a mix of approximately 50% SJL (CD45.1+) and 50% C57BL/6 (CD45.2+) splenocytes was prepared for the positive selection of CD45.1+ cells using the MojoSort™ Mouse CD45.1 Selection Kit. Left plot: CD45.1+ cells after isolation. Right plot: Cells before isolation. Cells were stained with anti-mouse CD45.1 (clone A20) APC and CD45.2 (clone 104) PE. Dead cells were excluded by Helix NP™ Blue.
Cat # Size Price Quantity Avail. Save
480117 20 tests $210
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480118 200 tests $595
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Description

Mouse CD45.1+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-mouse CD45.1 antibody and then magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD45.1+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat. No. 480117:

  • 1 vial containing 200 µl of Biotin anti-mouse CD45.1 antibody (clone A20)
  • 1 vial containing 200 µl Streptavidin Nanobeads


For Cat. No. 480118:

  • 2 vials containing 1 ml each of Biotin anti-mouse CD45.1 antibody (clone A20)
  • 2 vials containing 1 ml each of Streptavidin Nanobeads

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Formulation
Antibody: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
The antibodies were purified by affinity chromatography, and conjugated wtih biotin under optimal conditions. The solution is free of unconjugated biotin.
Streptavidin nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

Volume of Streptavidin Nanobeads should be adjusted depending on the starting percentage of CD45.1+ cells to be isolated. Use the table below as an example when working with a mixture of SJL (CD45.1+) and C57BL/6 (CD45.2+) mouse splenocytes. For 1x107 cells in 100 µl of buffer, use the following volumes:
 

SJL + C57BL/6 Mixture (spleen)

Approximate Starting CD45.1  Frequency

Optimal Nanobeads Volume

10% SJL + 90% C57BL/6

10-20%

2 µl

25% SJL + 75% C57BL/6

25-40%

2 µl

50% SJL + 50% C57BL/6

45-60%

10 µl

75% SJL + 25% C57BL/6

65-80%

10 µl

100% SJL

85-100%

20 µl


The volumes indicated in the table are for the use of MojoSort™ magnet (Cat. Nos. 480019/480020). For low frequency cells, pre-dilute the Streptavidin Nanobeads in order to pipette a minimum of 5 µl of any solution. For example, to isolate CD45.1+ cells from a mixture of 25% SJL and 75% C57BL/6, pre-dilute 10 µl of Streptavidin Nanobeads in 40 µl of MojoSort™ buffer (Cat. No. 480017) and add 10 µl of that dilution per sample. Avoid working with small volumes.

Application Notes

This kit is designed for the positive selection or depletion of mouse CD45.1+ cells from lymphoid tissue.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.


Antibody or cocktail dilution to use in column: 15X*
Nanobead dilution to use in columns: 4X*

*Note: Suggested dilutions for column use above determined based on a starting cell population consisting of a 1:1 mix of CD45.1+ and CD45.2+ cells. Further optimization may be necessary for different degrees of heterogeneity in starting cell populations.

Antigen Details

Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 2    Revision Date: 10/17/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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