MojoSort™ Mouse CD45 Nanobeads

Pricing & Availability
Other Names
Mouse CD45 Positive Selection, CD45 Positive Selection
Ave. Rating
2 reviews
Product Citations
publications
MCD45Nano_1_100215
A single cell suspension from collagenase enzymatically digested C57BL/6 mouse lung tissues was prepared to isolate CD45+ cells using the MojoSort™ Mouse CD45 Nanobeads. Cells were stained with CD45 (clone 30-F11) Alexa Fluor® 647. Dead cells were excluded by 7-AAD.
  • MCD45Nano_1_100215
    A single cell suspension from collagenase enzymatically digested C57BL/6 mouse lung tissues was prepared to isolate CD45+ cells using the MojoSort™ Mouse CD45 Nanobeads. Cells were stained with CD45 (clone 30-F11) Alexa Fluor® 647. Dead cells were excluded by 7-AAD.
  • MCD45Nano_2_100215
Cat # Size Price Quantity Avail. Save
480027 20 tests $150
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480028 200 tests $525
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Description

CD45+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD45 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD45+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480027

  • 1 vial containing 200 µl of CD45 Nanobeads

For Cat# 488028

  • 2 vials containing 1 ml each of CD45 Nanobeads

Product Details

Reactivity
Mouse
Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Antibody-conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
Mouse CD45 Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl Mouse CD45 Nanobeads in 1x107 in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection or depletion of CD45+ cells from lymphoid or different organ tissues. For depletion of CD45+ cells a higher volume of beads (up to 15 µl) and two separations may be required. Please contact technical support if needed.


Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 10X

Product Citations
  1. Navabi N, et al. 2017. Cell Reports. 10.1016/j.celrep.2017.04.046. PubMed
Publication Library

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD45 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 10/02/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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