MojoSort™ Mouse CD4 Nanobeads

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Other Names
Mouse CD4 Positive Selection, CD4 Positive selection
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MojoSort_Mouse_CD4_Nanobeads_1_122216
A single cell suspension from C57BL/6 mouse spleen was prepared to isolate CD4+ cells using the MojoSort™ Mouse CD4 Nanobeads. Cells were stained with CD4 (RM4-4) PE. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_CD4_Nanobeads_1_122216
    A single cell suspension from C57BL/6 mouse spleen was prepared to isolate CD4+ cells using the MojoSort™ Mouse CD4 Nanobeads. Cells were stained with CD4 (RM4-4) PE. Dead cells were excluded by 7-AAD.
  • MojoSort_Mouse_CD4_Nanobeads_2_122216
Cat # Size Price Quantity Avail. Save
480069 20 tests $170
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480070 200 tests $550
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Description

CD4+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD4 Nanobeads, after blocking with rat serum. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD4+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480069

  • 1 vial containing 200 µl of Mouse CD4 Nanobeads
  • 1 vial containing 100 µl of Rat serum

For Cat# 480070

  • 2 vials containing 1 ml each of Mouse CD4 Nanobeads
  • 1 vial containing 1 ml of Rat serum

Product Details

Reactivity
Mouse
Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Antibody-conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
Mouse CD4 Nanobeads should be stored undiluted between 2°C and 8°C.
Rat serum should be stored at -20°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

5 µl of Rat serum for 1x107 cells in 100 µl of buffer.
10 µl of Mouse CD4 Nanobeads for 1x107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection or depletion of mouse CD4+ cells from lymphoid tissues.

 

To find out the dilution to use this product in separation columns please download the CoA.

 

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD4 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 12/22/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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