- Other Names
- MojoSort™ Human CD8 Memory T Cell Isolation Kit
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PBMCs were prepared to isolate CD8+ Memory T cells using the MojoSort™ Human CD8 Memory T Cell Isolation Kit. The first dot plot shows T cells after isolation stained with CD8 (clone SK1) Brilliant Violet 421™ and CD3 (clone UCHT1) APC/Cy7. The second plot shows memory T cells after isolation, defined by CD45RO (clone UCHL1) PE and CD45RA (clone HI100) APC. Events shown were not previously gated on CD8+CD3+ cells. The third dot plot shows CD8+CD3+ cells before isolation. Dead cells were excluded by 7-AAD. -
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| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 480065 | 20 tests | $195 | ||||
| 480066 | 200 tests | $630 | ||||
CD8 Memory T cells are depleted by incubating the sample with the Human CD8 Memory T cell isolation biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched human CD8 Memory T cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Product DetailsKit Contents
- Kit Contents
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For Cat# 480065:
- 200 µl of Biotin-Antibody Cocktail: Biotin anti- CD4, CD14, CD16, CD19, CD20, CD36, CD45RA, CD56, CD123, CD235ab, TCR γ/δ, TCR Vδ2
- 200 µL of Streptavidin Nanobeads
For Cat# 480066:
- 2 vials of 1 ml each of Biotin-Antibody Cocktail each: Biotin anti- CD4, CD14, CD16, CD19, CD20, CD36, CD45RA, CD56, CD123, CD235ab, TCR γ/δ, TCR Vδ2
- 2 vials of 1 ml each of Streptavidin Nanobeads
Product Details
- Reactivity
- Human
- Formulation
- Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
- Preparation
- The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
- Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 µl of antibody cocktail for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer. - Application Notes
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This kit is designed for the isolation of untouched CD8 Memory T cells from peripheral blood mononuclear cells (PBMCs).
Antibody or cocktail dilution to use in column: 6X
Nanobead dilution to use in columns: 2X
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- NA
Related FAQs
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- Can your magnetic particles be sterile filtered?
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Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Is there a way to detach your magnetic particles from the cell surface?
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No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
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The average diameter is approximately 130 nm.
