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In this two-step kit, monocytes are first depleted by incubating PBMCs with biotin anti-human CD14 antibody, followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator; the unlabeled fraction is collected in a clean tube. In the second step, CD4+ T cells are positively selected from the previous unlabeled fraction by incubating it with directly conjugated Human CD4 Nanobeads. The second magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD4+ T cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Product Details
- Kit Contents
- 1 vial containing 1 ml of biotin anti-human CD14 antibody
- 2 vials containing 1 ml each of Streptavidin Nanobeads
- 2 vials containing 1 ml each of Human CD4 Nanobeads
- Verified Reactivity
Antibody: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2 with stabilizer.
Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Conjugated particles: protein-coated magnetic beads.
- Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
5 µl of biotin anti-human CD14 antibody for 1x107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer.
10 µl Human CD4 Nanobeads for 1 x 107 cells in 100 µl of buffer.
- Application Notes
This is a two-step kit designed for the positive selection of human CD4+ T cells from peripheral blood mononuclear cells (PBMCs), after the depletion of CD14+ cells.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
To find out the dilution to use this product in separation columns please download the CoA.
- Additional Product Notes
View more applications data for this product in our Scientific Poster Library.
- Biology Area
- Molecular Family
- CD Molecules
- Gene ID
- View information about CD4 on UniProt.org
- Is there a way to detach your magnetic particles from the cell surface?
No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
The average diameter is approximately 130 nm.
- Can your magnetic particles be sterile filtered?
Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
Please contact our technical service team for further assistance.