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Non-CD4+ Naïve T cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD4+ Naïve T cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Product Details
- Kit Contents
For Cat# 480041:
- 200 µl Biotin-Antibody Cocktail
- 200 µl Streptavidin Nanobeads
For Cat# 480042:
- 2 vials of 1 ml Biotin-Antibody Cocktail each
- 2 vials of 1 ml Streptavidin Nanobeads each
- Verified Reactivity
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
- Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
10 µl of antibody cocktail for 1 x 10^7 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 10^7 cells in 100 µl of buffer.
- Application Notes
This kit is designed for the isolation of untouched naïve CD4+ T cells from peripheral blood mononuclear cells (PBMCs).
This kit is designed for the MojoSort™ Isolation Kits No Wash Protocol. However, if one wash is added after the incubation with Streptavidin Nanobeads the purity increases about 2 - 4%. Typically without the wash the purity ranges from 91 - 96%. With one wash after the Streptavidin Nanobeads incubation the purity ranges from 93 - 98%. Purity is reported relative to target cells not previously gated on CD4+ cells.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 15X
Nanobead dilution to use in columns: 3.6X
- Additional Product Notes
View more applications data for this product in our Scientific Poster Library.
- Biology Area
- Molecular Family
- CD Molecules
- Gene ID
- View information about CD4 on UniProt.org
- Is there a way to detach your magnetic particles from the cell surface?
No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
The average diameter is approximately 130 nm.
- Can your magnetic particles be sterile filtered?
Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
Please contact our technical service team for further assistance.