MojoSort™ Human CD14 Selection Kit

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Other Names
CD14 Depletion, CD14 Enrichment, CD14 Positive Selection
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CD14_MojoSort_CD14_SelectionKit_1_111615
PBMCs were prepared to isolate CD14+ cells using the MojoSort™ Human CD14 Selection Kit. Cells were stained with CD14 PE (clone HCD14). Dead cells were excluded by 7-AAD.
  • CD14_MojoSort_CD14_SelectionKit_1_111615
    PBMCs were prepared to isolate CD14+ cells using the MojoSort™ Human CD14 Selection Kit. Cells were stained with CD14 PE (clone HCD14). Dead cells were excluded by 7-AAD.
  • CD14_MojoSort_CD14_SelectionKit_2_111615
Cat # Size Price Quantity Avail. Save
480025 20 tests $175
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480026 200 tests $560
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Description

CD14+ cells are either selected or depleted by incubating your sample with the biotin conjugated anti-human CD14 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD14+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. CD14 is expressed at high levels on human monocytes and macrophages and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cell, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in pathogen clearance and upregulation of adhesion molecules and cytokine expression. Clone 63D3 has been reported not to block LPS induced signal.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480025:

  • 1 vial containing 200 µl of biotin anti-human CD14 antibody (clone 63D3)
  • 1 vial containing 200 µl Streptavidin Nanobeads


For Cat# 480026

  • 2 vials containing 1 ml each of biotin anti-human CD14 antibody (clone 63D3)
  • 2 vials containing 1 ml each of Streptavidin Nanobeads

Product Details

Reactivity
Human
Formulation
Antibody: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.

Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated wtih biotin under optimal conditions. The solution is free of unconjugated biotin.

Storage & Handling
Antibody and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of antibody for 1x107 cells in 100 µl of buffer.

10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection or depletion of CD14+ cells from peripheral blood mononuclear cells (PBMCs).

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 16X
Nanobead dilution to use in columns: 3X

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD14 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 11/17/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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