MojoSort™ Human CD14+ Monocytes Isolation Kit

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Other Names
Human CD14 Monocyte Enrichment
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A_MojoSort_Human_CD14+_Monocytes_Isolation_Kit_1_020917_resized.jpg
PBMCs were prepared to isolate CD14+CD16-- monocytes using the MojoSort™ Human CD14+ Monocyte Isolation Kit. The first dot plot shows CD14+CD16- monocytes after isolation analyzed directly (no parent gate) and the second dot plot shows CD14+CD16- monocytes previously gated on CD45+ cells. The third dot plot shows cells before isolation. Cells were stained with CD16 (clone B73.1) PE and CD14 (clone HCD14) FITC. Dead cells were excluded by 7-AAD.
  • A_MojoSort_Human_CD14+_Monocytes_Isolation_Kit_1_020917_resized.jpg
    PBMCs were prepared to isolate CD14+CD16-- monocytes using the MojoSort™ Human CD14+ Monocyte Isolation Kit. The first dot plot shows CD14+CD16- monocytes after isolation analyzed directly (no parent gate) and the second dot plot shows CD14+CD16- monocytes previously gated on CD45+ cells. The third dot plot shows cells before isolation. Cells were stained with CD16 (clone B73.1) PE and CD14 (clone HCD14) FITC. Dead cells were excluded by 7-AAD.
  • B_MojoSort_Human_CD14+_Monocytes_Isolation_Kit_2_020917_resized
  • C_MojoSort_Human_CD14+_Monocytes_Isolation_Kit_3_020917_resized
Cat # Size Price Quantity Avail. Save
480047 10 tests $215
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480048 100 tests $650
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Description

Cell populations other than CD14+CD16- monocytes are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched monocytes are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480047:

  • 1 vial containing 100 µL of Biotin-Antibody Cocktail: Biotin anti- CD3, CD7, CD15, CD16, CD19, CD20, CD56, CD123, CD235ab
  • 1 vial containing 100 µL of Streptavidin Nanobeads
  • 1 vial containing 50 µL of Human TruStain FcX™ (Fc Receptor Blocking Solution)

For Cat# 480048:

  • 1 vial containing 1 mL of Biotin-Antibody Cocktail: Biotin anti- CD3, CD7, CD15, CD16, CD19, CD20, CD56, CD123, CD235ab
  • 1 vial containing 1 mL of Streptavidin Nanobeads
  • 1 vial containing 500 µL of Human TruStain FcX™ (Fc Receptor Blocking Solution)

Product Details

Reactivity
Human
Formulation
Cocktail: Phophate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin. Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail, Streptavidin Nanobeads, and Human TruStain FcX™ (Fc Receptor Blocking Solution) should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of antibody cocktail for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer.
5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution) for 1 x 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of untouched CD14+CD16- monocytes from peripheral blood mononuclear cells (PBMCs).
 

Antibody or cocktail dilution to use in column: 6X
Nanobead dilution to use in columns: 3X

Antigen Details

Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about Monocytes on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 0    Revision Date: 03/14/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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