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LEGENDplex™ Mouse Inflammation Panel (13-plex) with Filter Plate

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-23, IL-1α, IFN-γ, TNF-α, CCL2 (MCP-1), IL-12p70, IL-1β, IL-10, IL-6, IL-27, IL-17A, IFN-β, GM-CSF
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Product Citations
publications
Cat # Size Price Quantity Save
740150 100 tests $1761
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Description

Cytokines are small soluble glycoproteins that act as extracellular signaling molecuels and meidate communications between cells. They regulate immunity, inflammation and hematopoiesis as well as cell growth, migration, development and differentiation. Cytokines act in large tightly regulated networks where both the production and action of one cytokine is affected by the behavior of other cytokines. The accurate measurement of the expression profile of cytokines in a sample is critical to complete and in-depth understanding of disease progression and immune responses.


The LEGENDplex™ Mouse Inflammation Panel is a multiplex assay by using fluorescence–encoded beads suitable for the analysis on various flow cytometers. This panel allows simultaneous quantification of 13 mouse cytokines, including IL-1α, IL-1β, IL-6, IL-10, IL-12p70, IL-17A, IL-23, IL-27, MCP-1, IFN-β, IFN-γ, TNF-α, and GM-CSF. Most of cytokines in this panel are produced by innate immune cells, linking the innate and adaptive immunity and/or bystander cells. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples.

A LEGENDplex™ Mix and Match system is available to customize assays for specificities within this panel.

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads: PE Beads (1 mL)
  • Setup Beads: Raw Beads (2 mL)
  • Capture Beads
  • Detection Antibody (3.5 mL)
  • Standard Cocktail, Lyophilized (1 vial)
  • SA-PE (3.5 mL)
  • Matrix C, Lyophilized (1 vial)
  • Assay Buffer (25 mL)
  • Wash Buffer (25 mL)
  • Filter Plate (1 plate)
  • Plate Sealers (4 sheets)

Product Details

Verified Reactivity
Mouse
Application

Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Osterloh A, et al. 2016. Infect Immun . . PubMed
  2. Chalmers SA, et al. 2016. Clin Immunol. . PubMed
  3. Yang L, et al. 2022. BMC Cardiovasc Disord. 22:61. PubMed
  4. Mendes A, et al. 2021. Life Sci Alliance. 4:00. PubMed
  5. Colby JK, et al. 2016. Am J Pathol. . PubMed
  6. Hörhold F, et al. 2020. PLoS Comput Biol. 16:e1007657. PubMed
  7. Hirakawa MP, et al. 2022. iScience. 25:103759. PubMed
  8. Sharma NS, et al. 2020. J Clin Invest. 130:451. PubMed
  9. Abou Saleh L, et al. 2021. FASEB J. 35:e21797. PubMed
  10. Papp S, et al. 2016. PLoS Negl Trop Dis. . PubMed
  11. Leite Dantas R, et al. 2017. Am J Pathol. 187:2388. PubMed
  12. Blank J, et al. 2016. Front Microbiol. . PubMed
  13. Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
  14. Liu S, et al. 2017. J Pharmacol Sci. 10.1016/j.jphs.2017.02.001. PubMed
  15. Maueröder C, et al. 2016. Cell Death Differ. . PubMed
  16. Eastman AJ, et al. 2019. Sci Adv. 5:eaaw9051. PubMed
  17. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  18. Kastelberg B, et al. 2020. PLoS Pathog. 16:e1008854. PubMed
  19. Singh Y, et al. 2020. Sci Rep. 10:16131. PubMed
  20. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  21. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  22. Saveljeva S, et al. 2022. Cell Metab. 34:106. PubMed
  23. Hargrave KE, et al. 2019. Front Cell Infect Microbiol. 0.589583333. PubMed
  24. Wurster JI, et al. 2021. Cell Rep. 37:110113. PubMed
  25. Agliano F, et al. 2022. iScience. 25:103932. PubMed
  26. Li S, et al. 2021. Front Pharmacol. 12:634115. PubMed
  27. Blank J, et al. 2015. Infect Immun . . PubMed
  28. Borufka L, et al. 2016. Oncotarget. . PubMed
  29. Wang X, et al. 2021. Cell Death Dis. 12:402. PubMed
Materials Not Included
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes
  • Polypropylene Microfuge Tubes
  • Vortex Mixer
  • Sonicator
  • Plate Shaker
  • A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 5    Revision Date: 03/28/2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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