- Other Names
- Cytokine Release Syndrome, Cytokines, Multiplex, Flow Cytometry, LEGENDplex, CCL2, IL-10, CCL4 (MIP-1β), IFN-α, CXCL9 (MIG), CXCL10 (IP-10), TNF-α, IL-6, VEGF, IL-4, CCL3 (MIP-1α), IFN-γ, GM-CSF
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- Product Citations
741026, The LEGENDPlex™ Mouse Cytokine Release Syndrome Panel Standard contains 13 anti-human proteins against IFN- γ, IL-10, CCL4 (MIP-1β), IFN-α, CXCL9 (MIG), CXCL10 (IP-10), TNF-α, IL-6, VEGF, IL-4, CCL3 (MIP-1α), CCL2 (MCP-1), and GM-CSF. This Standard cocktail is intended for use with the following Mix and Match assay reagents:
LEGENDplex™ Mouse Cytokine Release Syndrome Panel
740065 LEGENDplex™ Mouse IFN-γ Capture Bead, A4, 13X
741013 LEGENDplex™ Mouse IL-10 Capture Bead A5, 13X
741014 LEGENDplex™ Mouse CCL4 (MIP-1β) Capture Bead A6, 13X
741015 LEGENDplex™ Mouse IFN-α Capture Bead A7, 13X
741016 LEGENDplex™ Mouse CXCL9 (MIG) Capture Bead A8, 13X
741017 LEGENDplex™ Mouse CXCL10 (IP-10) Capture Bead A10, 13X
741018 LEGENDplex™ Mouse TNF-α Capture Bead B2, 13X
740860 LEGENDplex™ Mouse IL-6 Capture Bead B3, 13X
741019 LEGENDplex™ Mouse VEGF Capture Bead B4, 13X
741020 LEGENDplex™ Mouse IL-4 Capture Bead B5, 13X
741021 LEGENDplex™ Mouse CCL3 (MIP-1α) Capture Bead B6, 13X
741012 LEGENDplex™ Mouse CCL2 (MCP-1) Capture Bead B7, 13X
740163 LEGENDplex™ Mouse GM-CSF Capture Bead, B9, 13X
741025 LEGENDplex™ MU Cytokine Release Syndrome Panel Detection Abs
740623 LEGENDplex™ Buffer Set I
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-bottom Plate
- Biology Area
- Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.