|740830||100 tests (3.5 mL)||$250|
The LEGENDplex™ Mouse B cell Panel Detection Antibodies contains 13 anti-mouse antibodies against IL-4, IL-6, IL-12p70, IL-17A, IL-2, TNF-α, Free Active TGF-β1, IL-13, IFN-γ, BAFF, BCMA, sCD40L, and IL-10. This Detection cocktail is recommended for use with the following in Mix and Match assays:
Mouse B cell Panel
740831 LEGENDplex™ Mouse B cell Panel Standard
740832 LEGENDplex™ Mouse IL-4 Capture Bead A4, 13X
740833 LEGENDplex™ Mouse IL-6 Capture Bead A5, 13X
740834 LEGENDplex™ Mouse IL-12p70 Capture Bead A6, 13X
740835 LEGENDplex™ Mouse IL-17A Capture Bead A7, 13X
740836 LEGENDplex™ Mouse IL-2 Capture Bead A8, 13X
740837 LEGENDplex™ Mouse TNF-α Capture Bead A10, 13X
740838 LEGENDplex™ Mouse Free Active TGF-β1 Capture Bead B2, 13X
740839 LEGENDplex™ Mouse IL-13 Capture Bead B3, 13X
740840 LEGENDplex™ Mouse IFN-γ Capture Bead B4, 13X
740841 LEGENDplex™ Mouse BAFF Capture Bead B5, 13X
740842 LEGENDplex™ Mouse BCMA Capture Bead B6, 13X
740843 LEGENDplex™ Mouse sCD40L Capture Bead B7, 13X
740844 LEGENDplex™ Mouse IL-10 Capture Bead B9, 13X
740373 LEGENDplex™ Buffer Set B
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-bottom Plate
- Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.