LEGENDplex™ Human IGFBP-4 Capture Bead B4, 13X

Pricing & Availability
Other Names
Insulin Like Growth Factor Binding Protein 4, IGF-Binding Protein 4, IBP-4, HT29-IGFBP, BP-4
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Cat # Size Price  
740476 100 tests (270 µL) $100
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To purchase, view our LEGENDplex™ Mix and Match page

Description

This Human IGFBP-4 Capture Bead is suitable for use in the following Mix and Match Panels:
 

LEGENDplex™ Human IGFBP Mix and Match Panel
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740471 (LEGENDplex™ Human IGFBP Panel Detection Antibodies)
740470 (LEGENDplex™ Human IGFBP Panel Standard)
740374 (LEGENDplex™ Buffer Set C)

The assembled Human IGFBP Mix and Match Panel is a multiplex bead-based assay panel that uses fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of up to 7 IGFBPs: IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGFBP-7, and IGFALS. This assay panel provides broader dynamic ranges than traditional ELISA methods, and has been validated for use with serum, plasma, cell culture supernatant, and other biological fluids samples.


LEGENDplex™ Human Vascular Inflammation Mix and Match Panel 1
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740553 (LEGENDplex™ Human Vascular Inflammation Panel 1 Detection Antibodies)
740554 (LEGENDplex™ Human Vascular Inflammation Panel 1 Standard)
740374 (LEGENDplex™ Buffer Set C)
740377 or 740379 (Filter Plate or V-bottom Plate)


The assembled Human Vascular Inflammation Mix and Match Panel 1 is a multiplex bead-based assay kit, using fluorescence–encoded beads suitable for use on various flow cytometers. This allows simultaneous quantification of up to 13 human proteins: Myoglobin, MRP8/14, NGAL, CRP, MMP-2, OPN, MPO, SAA, IGFBP-4, ICAM-1, VCAM-1, MMP-9, and Cystatin C. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for use with serum, plasma, tissue culture supernatant, and urine samples.

Product Details
View Current Manual (pdf)

Product Details

Reactivity
Human
Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.

Antigen Details

Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability
Gene ID
3487 View all products for this Gene ID

Related FAQs

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

If I don't have a vacuum, how do I remove the liquid from my plate?

Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are valid for 6 months from the date of receipt. 

Go To Top Version: 0    Revision Date: 02/10/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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