LEGENDplex™ Human Bone Formation Panel (4-plex) with V-bottom Plate

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Other Names
Human Bone Formation Panel (4-plex)
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Product Citations
publications
1_740672_740673_Sensitivity
Sensitivity is defined as the mean minimum detectable concentration (MDC) established by applying a 5-PL curve-fitting algorithm to assay curves run in both serum/plasma matrix (n = 13) or cell culture media (n = 11) in the LEGENDplex™ Data Analysis Software.
  • 1_740672_740673_Sensitivity
    Sensitivity is defined as the mean minimum detectable concentration (MDC) established by applying a 5-PL curve-fitting algorithm to assay curves run in both serum/plasma matrix (n = 13) or cell culture media (n = 11) in the LEGENDplex™ Data Analysis Software.
  • 2_740672_740673_Representative-Curves
    Representative LEGENDplex™ Human Bone Metabolism Panel 1 standard curves. Data were acquired using a dual laser BD FACSCalibur™ flow cytometer.
  • 3_740672_740673_Biological-Detectability
    Synovial fluid samples from patients with psoriatic arthritis (PsA, n = 24) or rheumatoid arthritis (RA, n = 22) were quantified using the LEGENDplex™ Human Bone Metabolism Panel 1. Data are presented as Mean + SD. Data were provided courtesy of Dr. Erik Lubberts, Erasmus University Medical Center, Rotterdam, Netherlands.
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740673 100 tests $600
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Description

Bone metabolism is a complex equilibrium between bone formation and resorption regulated through numerous hormones, steroids, growth factors, and cytokines. Bone formation is mediated through specialized cells called osteoblasts, while resorption is carried out via osteoclasts. Bone metabolism is a constant, life-long process that exists in balance. Disruptions to the homeostasis between formation and resorption results in diseases such as: osteoporosis, osteopetrosis, Paget’s disease, rickets, and renal osteodystrophy.

The LEGENDplex™ Human Bone Formation Panel (4-plex) is a bead-based multiplex assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. It allows for simultaneous quantification of 4 key targets involved in bone formation including OPG, ALPL, Leptin, and BMP-2. This assay panel provides higher detection sensitivity and broader dynamic range than traditional ELISA methods. The panel has been validated for use on cell culture supernatant, serum, and plasma samples.

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads 1: FITC Beads (1 ml)
  • Setup Beads 2: PE Beads (1 ml)
  • Setup Beads 3: Raw Beads (1 ml)
  • Capture Beads (varies)
  • Detection Antibodies (3.5 ml)
  • Standard Cocktail, Lyophilized (1 vial)
  • SA-PE (3.5 ml)
  • Matrix C, Lyophilized (1 vial)
  • Reconstitution Buffer (1 ml)
  • Assay Buffer (25 ml)
  • Wash Buffer (25 ml)
  • Plate Sealers (4 sheets)
  • Data Analysis Software Dongle
  • Filter Plate
  • Manual

Product Details

Reactivity
Human
Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex.

Download the LEGENDplex™ software here.
Materials Not Included
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes
  • Polypropylene Microfuge Tubes
  • Vortex Mixer
  • Aluminum foil
  • Absorbent pads or paper towels
  • Sonicator
  • Plate Shaker
  • Tabletop centrifuge
  • A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)

Antigen Details

Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines, Growth Factors, Enzymes and Regulators
Gene ID
4982 View all products for this Gene ID 249 View all products for this Gene ID 3952 View all products for this Gene ID 650 View all products for this Gene ID

Related FAQs

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

If I don't have a vacuum, how do I remove the liquid from my plate?

Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are valid for 6 months from the date of receipt. 

Go To Top Version: 2    Revision Date: 04/13/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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