LEGEND MAX™ Mouse IFN-β ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-GI factor, IFN-β Pre-coated ELISA Kit
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IFNb_Mouse_leg_070612
  • IFNb_Mouse_leg_070612
Cat # Size Price Quantity Avail. Save
439407 1 Pre-coated Plate $495
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Description

Interferon-β is a member of the type I interferon class. Type I IFNs are involved in the innate immune system, being released non-specifically during the early stage of infection in response to stimuli such as viruses, dsRNA, LPS, and bacterial components. Once secreted by virus-infected or activated cells, they bind to the IFNα/β receptor (IFNAR), which is found on almost all cell types. Then, via the JAK-STAT pathway, they induce the expression of hundreds of IFN-stimulated genes (ISGs). While the function of many of these proteins remains to be elucidated, a hallmark of type I IFNs is their anti-viral activities, both general and specific against particular viruses. Type I IFNs also regulate cell growth and apoptosis, and exert various immunomodulatory and inflammatory effects. As such, they are clinically relevant, both as therapeutics and as targets of inhibition for the treatment of various autoimmune diseases and cancer.


Mouse IFN-β is a ~20 kD monomeric glycoprotein. It has 50% amino acid sequence homology with mouse IFN-α, its closest related protein, and 62% homology with human IFN-β. IFN-β is currently the therapeutic of choice for treating multiple sclerosis.

BioLegend’s LEGEND MAX™ Mouse IFN-β ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a specific antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Mouse IFN-β ELISA Kit is specifically designed for the accurate quantitation of mouse IFN-β from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Mouse IFN-β Pre-coated 96-well Strip Microplate
  • Mouse IFN-β Dectection Antibody
  • Mouse IFN-β Standard
  • Avidin-HRP D
  • Matrix C (for serum and plasma samples only)
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Mouse
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Shah S, et al. 2013. J. Immunol. 191:3514. PubMed
  2. Wang S, et al. 2013. J Virol. 87:11851. PubMed
Product Citations
  1. Oldenburg R, et al. 2018. Front Immunol. 9:2. PubMed
  2. Zhou P, et al. 2018. Nat Commun. 9:1243. PubMed
  3. Cheng WY, et al. 2018. Front Immunol. 9:1297. PubMed
  4. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  5. Yan S, et al. 2018. J Immunol. 200:1016. PubMed
  6. Yang S, et al. 2019. Sci Adv. 5:eaav7999. PubMed
  7. Yoshida A, et al. 2018. J Virol. 92:e02094. PubMed
  8. Kwon HK, et al. 2019. Exp Mol Med. 51:50. PubMed
  9. Fritzlar S, et al. 2019. MBio. 10:e00960. PubMed
  10. Stavrou S, et al. 2018. MBio. 9:e00923. PubMed
  11. Srikanth S, et al. 2019. Nat Immunol. 20:152. PubMed
  12. Sousa J, et al. 2020. Nat Commun. 11:1949. PubMed
  13. Shah S, et al. 2013. J Immunol. 191:3514. PubMed
  14. Wang S, et al. 2013. J Virol. 87:11851. PubMed
  15. Zhu M, et al. 2015. J Immunol. 195: 289 - 297. PubMed
  16. Lv M, et al. 2015. Sci Rep. 5: 12954. PubMed
  17. Ferri F, et al. 2015. Nat Commun. 6: 8900. PubMed
  18. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
  19. Jiang H, et al. 2016. J Immunol. 197: 2880 - 2890. PubMed
  20. Wu Y, Ren D, Chen G 2016. J Immunol. 197: 3336 - 3347. PubMed
  21. Nanda S, et al. 2016. J Immunol. 197(11):4266-4273. PubMed
  22. Khan M, et al. 2020. PLoS Pathog. 16:e1009020. PubMed
  23. Piersma SJ, et al. 2020. Elife. 9:00. PubMed
  24. Karki R, et al. 2020. J Immunol. 204:2514. PubMed
  25. Reimer E, et al. 2020. J Cell Sci. 134:00:00. PubMed
Sensitivity
1.9 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Fibroblasts, epithelial cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Gene ID
15977 View all products for this Gene ID
UniProt
View information about IFN-beta on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 3    Revision Date: 01/16/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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