- Other Names
- Soluble GARP (Glycoprotein A Repetitions Predominant), Soluble LRRC32 (Leucine-Rich Repeat Protein 32)
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- Product Citations
|440107||1 Pre-coated Plate||$495|
GARP stands for Glycoprotein A Repetitions Predominant. It belongs to the leucine-rich repeat protein family and is also known as LRRC32. It is an 80 kD type I transmembrane protein. The gene sequence is only found in vertebrates, and human GARP shares 80% amino acid identity with mouse and rat GARP.
GARP presumably plays a large role in embryogenesis, since it is widely expressed across a broad range of tissue types during development. The R395W mutation is associated with Usher syndrome type I, which results in congenital deafness, vestibular dysfunction, and blindness.
In the adult, GARP is expressed predominantly by megakaryocytes, platelets, placenta, lung, kidney, and skeletal muscle. It was discovered that GARP is selectively expressed on naturally activated FOXP3+ Treg cells, providing a valuable marker for this cell type. There seems to be some interplay between GARP and FOXP3, such that expression of one influences the expression of the other. GARP also binds to latent TGF-ß on the cell surface via LAP, effectively controlling the activation of latent TGF-ß by keeping it sequestered to the cell surface. As such, GARP is involved in suppression and modulation of the immune response.
Transcript levels of GARP are also high in various cancers, such as squamous cell carcinoma, colorectal cancer, breast cancer, ovarian carcinoma, and prostate cancer. Because recombinant soluble GARP (sGARP) can bind to recombinant latent TGF-ß, and shows suppressive bioactivity in vitro, there is an intriguing possibility that the soluble form may exist naturally, and could be a biomarker for various cancers or autoimmune conditions.
BioLegend's LEGEND MAX™ Human Soluble GARP/LRRC32 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal capture antibody. This kit specifically quantitates human soluble GARP/LRRC32 from cell culture supernatant, serum, plasma and other biological fluids. It is analytically validated with ready-to-use reagents.
- Kit Contents
- Anti-Human sGARP/LRRC32 Pre-coated 96-well Strip Microplate
- Human sGARP/LRRC32 Dectection Antibody
- Human sGARP/LRRC32 Standard
- Avidin-HRP E
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Additional Product Notes
View more applications data for this product in our Scientific Poster Library.
- Product Citations
- 0.062 ng/mL
- Standard Range
- 0.16-10 ng/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?
We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.