- Other Names
- Fibroblast growth factor 2 (Fgf-2), Fgfb, bFGF, FGF-basic Precoated ELISA Kit
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- Product Citations
|434309||1 Pre-coated Plate||$350|
Fibroblast growth factor-basic (FGF-basic) is a heparin-binding growth factor which belongs to a family of fibroblast growth factors (FGFs). FGF family members share 35-60% amino acid sequence homology and are highly conserved among species. FGF-basic has been isolated from neural, pituitary, adrenal cortical, and placental tissues. Human FGF-basic is a 17.2 kD protein containing 154 amino acids. Other isofoms (22, 22.5, 24 and 34 kD) have also been reported with distinct intracellular localizations and functions. FGFs are involved in many physiological and pathological processes including embryonic development, neuronal outgrowth, angiogenesis, and malignant transformation. FGF-basic is essential for the development and maintenance of vascular integrity during embryogenesis and is also a major angiogenic factor involved in wound healing, cardiovascular disease, and tumor neovascularization.
BioLegend’s LEGEND MAX™ Human FGF-basic ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a human FGF-basic specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human FGF-basic ELISA Kit is specifically designed for the accurate quantitation of human FGF-basic from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Kit Contents
- Anti-Human FGF-basic Pre-coated 96-well Strip Microplate
- Human FGF-basic Dectection Antibody
- Human FGF-basic Standard
- Matrix A
- Avidin-HRP E
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- 4 pg/mL
- Standard Range
- 12.5 - 800 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- Cell Sources
- Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, epithelial cells
- Biology Area
- Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Gene ID
- 2247 View all products for this Gene ID
- View information about FGF-basic on UniProt.org
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?
We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.