LEGEND MAX™ Human CCL28 ELISA Kit

Pricing & Availability
Other Names
Human CCL28 Precoated ELISA Kit, Mucosae-associated epithelial chemokine (MEC), CCK1, SCYA28, Chemokine (C-C motif) ligand 28
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CCL28_Human_ELISA_Kit_LegMax_013014
  • CCL28_Human_ELISA_Kit_LegMax_013014
Cat # Size Price Quantity Save
441207 1 Pre-coated Plate $425
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Description

CCL28 is also known as mucosa-associated epithelial chemokine (MEC) and CCK1. It is a member of the C-C group of the chemokine family. The full-length human CCL28 protein consists of 127 amino acids and has a molecular weight of ~12 kD. Human CCL28 is closely related to CCL27 and shares 83% homology to mouse CCL28. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast, and salivary glands. The expression levels of CCL28 can be increased by several stimulants including pro-inflammatory cytokines, bacterial flagellin, and n-butyrate, a product of microbial metabolism.

CCL28 binds to G-protein coupled receptors CCR3 and CCR10, and induces calcium mobilization. It is involved in homing of T and B lymphocytes that express CCR10, and migration of eosinophils that express CCR3. CCL28 can also act as an antimicrobial protein, suggesting a direct contribution to innate immune protection. CCL28 has been implicated in a number of disease processes. Serum CCL28 levels have markedly increased in patients with atopic dermatitis. Increased expression of CCL28 is also associated with asthma, psoriasis, vulgaris, and bullous pemphigoid.

The LEGEND MAX™ Human CCL28 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human CCL28 antibody. The detection antibody is a biotinylated mouse monoclonal anti-human CCL28 antibody. This kit is specifically designed for the accurate quantitation of human CCL28 from cell culture supernatant, serum, plasma (EDTA and citrate only), and other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-CCL28 Pre-coated 96-well Strip Microplate
  • CCL28 Dectection Antibody
  • CCL28 Standard
  • Matrix A
  • Avidin-HRP A
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Sensitivity
5.3 pg/mL
Standard Range
7.8 - 500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
56477 View all products for this Gene ID
UniProt
View information about CCL28 on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 1    Revision Date: 01/30/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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