- Other Names
- Amyloid Beta A4 Protein, Prea4, Protease Nexin-II, Peptidase Nexin-II, Beta-Amyloid Peptide, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, APP, Amyloid Precursor Protein
- Previously
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Covance Catalog# SIG-38956
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity | Save | ||
|---|---|---|---|---|---|---|
| 842401 | 1 Pre-coated Plate | $575 | ||||
Kit Contents
- Kit Contents
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• Capture Antibody Coated Plate, 1 plate
• 1-42 Standard, 2 vials containing 20ug each
• 5X Wash Buffer, 125mL
• Standard Diluent, 2 x 3mL
• 2X Incubation Buffer, 12mL
• HRP Detection Antibody, 40uL
• TMB Substrate, 22mL
• Plate Sealer, 2
• 1.5mL Microfuge tubes, 20
Product Details
- Reactivity
- Human, Mouse, Rat
- Storage & Handling
- Store between 2°C and 8°C.
- Application
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ELISA
- Recommended Usage
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Please download the Colorimetric LEGEND MAX™ ELISA Kit Protocol for further assay detail.
- Application Notes
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This antibody kit is used to quantify β-Amyloid [x-42].
This kit has a guaranteed shelf life of 2 to 6 months.
BioLegend's Colorimetric LEGEND MAX™ x-42 kit is specific for the x-42 isoform of beta amyloid. It has negligible cross reactivity with the x-40 isoform of beta amyloid. The Colorimetric LEGEND MAX™ 42 kit reacts with both human and rodent x-42.
Please note that the name of this kit has changed from BetaMark™ β-Amyloid x-42 ELISA Kit (TMB) to LEGEND MAX™ β-Amyloid x-42 ELISA Kit with Pre-coated Plate. -
Application References
(PubMed link indicates BioLegend citation) -
- Thakker DR, et al. Intracerebroventricular amyloid-beta antibodies reduce cerebral amyloid angiopathy and associated micro-hemorrhages in aged Tg2576 mice. Proc Natl Acad Sci USA Feb 25, 2009.
- Modi KK, et al. 2015. PloS One. 10:130398. PubMed
- Product Citations
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- Standard Range
- 7.4 - 250 pg/mL
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid
- Gene ID
- 351 View all products for this Gene ID
- UniProt
- View information about beta-Amyloid on UniProt.org
Related FAQs
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
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Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
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The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?
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We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.