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LEAF™ Purified anti-human G-CSF Antibody

Pricing & Availability
Clone
Poly21A06 (See other available formats)
Regulatory Status
RUO
Other Names
Granulocyte-Colony Stimulating Factor, CSF3, CSF-beta
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly21A06_LEAF-Purified_G-CSF_Antibody_080922
Recombinant human G-CSF (Cat. No. 578604) (black circles) induces proliferation of M-NFS-60 cells. LEAF™ purified anti-human G-CSF (clone Poly21A06) (purple squares) neutralizes M-NFS-60 cell proliferation induced by recombinant human G-CSF at 0.6 ng/mL in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range is 0.02 - 0.1 µg/mL.
  • Poly21A06_LEAF-Purified_G-CSF_Antibody_080922
    Recombinant human G-CSF (Cat. No. 578604) (black circles) induces proliferation of M-NFS-60 cells. LEAF™ purified anti-human G-CSF (clone Poly21A06) (purple squares) neutralizes M-NFS-60 cell proliferation induced by recombinant human G-CSF at 0.6 ng/mL in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range is 0.02 - 0.1 µg/mL.
  • Poly21A06_ULEAF_G-CSF_Antibody_081022
    Whole cell extracts (15 µg total protein) were prepared from A431 cells spiked with or without 20 ng of recombinant human G-CSF protein (Cat. No. 578602). Proteins were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 0.5 µg/mL of LEAF™ purified anti-human G-CSF (clone Poly21A06) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat IgG (H+L) antibody at a 1:10,000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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521603 100 µg $421
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Description

Human G-CSF was cloned from a human squamous carcinoma cell line and is the key hematopoietic cytokine involved in the control of neutrophil production. Therefore, it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by downregulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrades important HSC trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; it is also expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human G-CSF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing proliferation induced by recombinant human G-CSF (Cat. No. 578604) at 0.6 ng/mL on M-NFS-60 cells. ND50 range: 0.02 - 0.1 µg/mL. For western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody has not been tested for ICC. 

RRID
AB_2927858 (BioLegend Cat. No. 521603)

Antigen Details

Structure
Heterodimer
Distribution

Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, epithelial cells, and bone marrow stromal cells

Function
G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxido anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
Interaction
Hematopoietic progenitor cells, neutrophilic granulocytes, monocytes, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, cardiac fibroblasts, neurons, and some small cell lung carcinoma cell lines
Ligand/Receptor
CD114 (G-CSFR)
Antigen References
  1. Nagata S, et al. 1986. Nature. 319:415.
  2. Kim HK, et al. 2006. Blood. 108:812.
  3. Donahue RE, et al. 2009. Blood. 114:2530.
  4. Shimoji K, et al. 2010. Cell Stem Cell. 6:227.
  5. Martins A, et al. 2010. IUBMB Life. 62:611.
  6. Hara M, et al. 2011. J Exp Med. 208:715.
Gene ID
1441 View all products for this Gene ID
UniProt
View information about G-CSF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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