- AFL4 (See other available formats)
- Other Names
- Vascular endothelial growth factor receptor 3, FLT-4, Fms-like tyrosine kinase 4
- Rat IgG2a, κ
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- Product Citations
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VEGFR-3, also known as FLT4, is a member of the Tyr protein kinase family. The extracellular portion of VEGFR-3 contains 7 immunoglobulin (Ig)-like domains and the cytoplasmic portion contains a protein kinase domain. FLT4 regulates angiogenesis and lymphangiogenesis, its ligands are VEGF-C and D and its binding is mediated by the 2nd and 3rd Ig-like domains of FLT4. During fetal development VEGFR-3 is expressed on endothelial cells, however, in the adult mice, the vascular endothelial cells lose VEGFR-3 expression, but the lymphatic endothelium expresses it constitutively. In addition, VEGFR-3 expression can be induced in tumors with active angiogenesis.Product Details
- Antibody Type
- Host Species
- VEGFR-3-Fc fusion protein
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
IHC - Quality tested
Block, ELISA, IP, WB - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining of mouse frozen tissue sections.
- Application Notes
Additional reported applications (for the relevant formats) include: ELISA1,2, immunoprecipitation1and Western blot1,2 from cell lysates, immunohistochemical staining1, 3 of frozen and paraffin embedded tissue sections and in whole-mount mouse embryos, in vivo blocking1, 5 of VEGF-C binding to inhibit angiogenesis, lymphangiogenesis, growth and metastasis of different tumor cells, in vitro blocking4 of VEGF-C binding to induce dispersion of endothelial cell clusters.
(PubMed link indicates BioLegend citation)
- Kubo H, et al. 2000. Blood 96:546. (IP, WB, IHC, ELISA, Block)
- Zhang L, et al. 2010. Cell. Res. Epub ahead of print (WB, ELISA)
- Tammela T, et al. 2010. Nature 454:656. (IHC)
- Shimizu K, et al. 2004. Cancer Sci. 95:328. (Block)
- Matsumura K, et al. 2003. Blood 101:1367. (Block)
AB_10683448 (BioLegend Cat. No. 140904)
- Member of Tyr protein kinase family. Extracellular portion contains 7 immunoglobulin-like domains. Ligand binding is mediated by its domains 2 and 3. Cytoplasmic portion contains 1 protein kinase domain.
- Expressed on vascular endothelial cells during fetal development, constitutively expressed on lymphatic endothelium in adult mice, induced in tumors with active angiogenesis.
- Regulates angiogenesis and lymphangiogenesis.
- Associated with VEGFR-2 in the surface.
- Ligand Receptor
- VEGF-C and D
- Cell Type
- Endothelial cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors, Protein Kinases/Phosphatase
- Antigen References
1. Uhrin P, et al. 2010. Blood 115:3997.
2. Wang Y, et al. 2010. Nature 465:483.
3. Akobsson L, et al. 2009. Biochem. Soc. T. 37:1233.
4. Tammela T, et al. 2010. Nature 454:656.
5. Huang G and Chen L. 2008. Cancer Biother. Radio. 23:661.
6. Zhang YJ and Fang JY. 2008. J. Gastroen. Hepatol. 23:856.
- Gene ID
- 14257 View all products for this Gene ID
- View information about VEGFR-3 on UniProt.org
- Does BioLegend test each LEAF™ antibody by functional assay?
No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house unless otherwise indicated. However upon request these can be tested from outside independent laboratories on custom basis.
- Does BioLegend test each LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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