LEAF™ Purified anti-mouse VEGFR-3 (FLT-4) Antibody

Pricing & Availability
Clone
AFL4 (See other available formats)
Other Names
Vascular endothelial growth factor receptor 3, FLT-4, Fms-like tyrosine kinase 4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
AFL4_Pure_1_111710
6 µm frozen thymus section from a naïve C57BL/6 mouse was stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green) and DAPI (blue). Magnification 40X.
  • AFL4_Pure_1_111710
    6 µm frozen thymus section from a naïve C57BL/6 mouse was stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green) and DAPI (blue). Magnification 40X.
  • AFL4_Pure_2_111710
    6 µm frozen lung section from an influenza-infected C57BL/6 mouse stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green), anti-CD3 (red) and DAPI (blue). Magnification 40X.
Cat # Size Price Quantity Avail. Save
140904 500 µg $300
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Description

VEGFR-3, also known as FLT4, is a member of the Tyr protein kinase family. The extracellular portion of VEGFR-3 contains 7 immunoglobulin (Ig)-like domains and the cytoplasmic portion contains a protein kinase domain. FLT4 regulates angiogenesis and lymphangiogenesis, its ligands are VEGF-C and D and its binding is mediated by the 2nd and 3rd Ig-like domains of FLT4. During fetal development VEGFR-3 is expressed on endothelial cells, however, in the adult mice, the vascular endothelial cells lose VEGFR-3 expression, but the lymphatic endothelium expresses it constitutively. In addition, VEGFR-3 expression can be induced in tumors with active angiogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
VEGFR-3-Fc fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

IHC - Quality tested
Block, ELISA, IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining of mouse frozen tissue sections.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA1,2, immunoprecipitation1and Western blot1,2 from cell lysates, immunohistochemical staining1, 3 of frozen and paraffin embedded tissue sections and in whole-mount mouse embryos, in vivo blocking1, 5 of VEGF-C binding to inhibit angiogenesis, lymphangiogenesis, growth and metastasis of different tumor cells, in vitro blocking4 of VEGF-C binding to induce dispersion of endothelial cell clusters.

Application References

(PubMed link indicates BioLegend citation)
  1. Kubo H, et al. 2000. Blood 96:546. (IP, WB, IHC, ELISA, Block)
  2. Zhang L, et al. 2010. Cell. Res. Epub ahead of print (WB, ELISA)
  3. Tammela T, et al. 2010. Nature 454:656. (IHC)
  4. Shimizu K, et al. 2004. Cancer Sci. 95:328. (Block)
  5. Matsumura K, et al. 2003. Blood 101:1367. (Block)
RRID
AB_10683448 (BioLegend Cat. No. 140904)

Antigen Details

Structure
Member of Tyr protein kinase family. Extracellular portion contains 7 immunoglobulin-like domains. Ligand binding is mediated by its domains 2 and 3. Cytoplasmic portion contains 1 protein kinase domain.
Distribution
Expressed on vascular endothelial cells during fetal development, constitutively expressed on lymphatic endothelium in adult mice, induced in tumors with active angiogenesis.
Function
Regulates angiogenesis and lymphangiogenesis.
Interaction
Associated with VEGFR-2 in the surface.
Ligand Receptor
VEGF-C and D
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, Protein Kinases/Phosphatase
Antigen References

1. Uhrin P, et al. 2010. Blood 115:3997.
2. Wang Y, et al. 2010. Nature 465:483.
3. Akobsson L, et al. 2009. Biochem. Soc. T. 37:1233.
4. Tammela T, et al. 2010. Nature 454:656.
5. Huang G and Chen L. 2008. Cancer Biother. Radio. 23:661.
6. Zhang YJ and Fang JY. 2008. J. Gastroen. Hepatol. 23:856.

Gene ID
14257 View all products for this Gene ID
UniProt
View information about VEGFR-3 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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