LEAF™ Purified anti-human CD290 (TLR10) Antibody

Pricing & Availability
Clone
3C10C5 (See other available formats)
Other Names
Toll like receptor 10
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3C10C5_LEAF_1_060412
Human peripheral blood lymphocytes were stained with TLR10 (clone 3C10C5, top) LEAF™ or mouse IgG1, κ isotype control (bottom) followed by anti-mouse IgG1 PE and CD20 PerCP.
  • 3C10C5_LEAF_1_060412
    Human peripheral blood lymphocytes were stained with TLR10 (clone 3C10C5, top) LEAF™ or mouse IgG1, κ isotype control (bottom) followed by anti-mouse IgG1 PE and CD20 PerCP.
  • 3C10C5_LEAF_2_060412
Cat # Size Price Quantity Avail. Save
354601 50 µg $175
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354602 500 µg $525
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Description

CD290, also known as TLR10, is a 91-100 kD glycosylated protein belonging to the toll-like receptor family. It is expressed on B cell, Treg cells, and plasmacytoid dendritic cells but absent on T cells, particularly non-Treg and granulocytes. It can interact with MyD88 and plays an important role in innate immunity and pattern recognition.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified extracellular domain of human TLR10 (amino acids 20-474) (NP_001017388 )
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Guan Y, et al. 2010. J. Immunol. 184:5094.
RRID
AB_11218611 (BioLegend Cat. No. 354601)
AB_11219401 (BioLegend Cat. No. 354602)

Antigen Details

Structure
Member of the toll-like receptor family, type I membrane protein, 91-100 kD
Distribution
B cells, Treg cells, plasmacytoid DCs
Function
Interacts with MyD88 and TRAF6, which results in NF-kB activation and cytokine secretion
Ligand/Receptor
MyD88 and TRAF6
Cell Type
B cells, Tregs, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Chuang T, et al. 2001. Biochim. Biophys. Acta. 1518:157.
2. Hornung V, et al. 2002. J. Immunol. 168:4531.
3. Bourke E, et al. 2003. Blood 102:956.

Gene ID
81793 View all products for this Gene ID
UniProt
View information about CD290 on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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