LEAF™ Purified anti-mouse VEGF-A Antibody

Pricing & Availability
Clone
2G11-2A05 (See other available formats)
Other Names
Vascular endothelial growth factor (VEGF)
Isotype
Rat IgG2a, κ
Ave. Rating
0 reviews
2G11-2A05_LEAF_VEGF-A_Antibody_WB_051016
Western blot analysis of 50 ng of recombinant mouse VEGF-164 (lane 1), recombinant mouse VEGF-120 (lane 2), recombinant human VEGF-165 (lane 3), recombinant human VEGF-121 (lane 4), and recombinant human VEGF-C (lane 5) using LEAF™ purified anti-mouse VEGF-A (clone 2G11-2A05) antibody.
  • 2G11-2A05_LEAF_VEGF-A_Antibody_WB_051016
    Western blot analysis of 50 ng of recombinant mouse VEGF-164 (lane 1), recombinant mouse VEGF-120 (lane 2), recombinant human VEGF-165 (lane 3), recombinant human VEGF-121 (lane 4), and recombinant human VEGF-C (lane 5) using LEAF™ purified anti-mouse VEGF-A (clone 2G11-2A05) antibody.
Cat # Size Price Quantity Avail. Save
512807 50 µg $135.00
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512808 500 µg $450.00
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Description

VEGF-A, also known as VEGF (vascular endothelial growth factor), is the founding member of the family including VEGF (VEGF-A), VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides with a highly conserved receptor-binding cystine-knot structure similar to that of the platelet-derived growth factors.VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis.VEGF-A is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. Oxygen tension is a key physiological regulator of VEGF-A gene expression.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human
Host Species
Rat
Immunogen
N-terminal 24 aa sequence of murine VEGF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The VEGF-A antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
Neut, ELISA, IHC, FA - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 2.5-10 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Neutralization: The LEAF™ Purified antibody is recommended for neutralization of mouse VEGF-A bioactivity in vivo2,4 and in vitro2.
Western Blotting: It is suggested to use 0.5-2 µg/ml for WB2.
Immunohistochemistry: Clone 2G11-2A05 is recommended for IHC on saponin-treated frozen tissue sections at a concentration range of 5-10 µg/ml.
ELISA or ELISPOT: The LEAF™ Purified 2G11-2A05 antibody is useful in a mouse VEGF-A ELISA or ELISPOT assay.
Functional Assay: The LEAF™ Purified 2G11-2A05 antibody is useful in a mouse VEGF-A functional assay2.


It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Wuest TR, et al. 2009. J. Exp Med. 207;101. (Neut) PubMed
  2. Basu A, et al. 2008. Cancer Res. 68:5689. (Neut, WB, FA)
  3. Kumar V, et al. 2010. Blood 115:4725. PubMed
  4. Lu R, et al. 2012. Cancer Res. 72:2239. (Neut) PubMed
  5. Jayarman P, et al. 2012. J. Immunol. 188:5365. PubMed
  6. Kather JN, et al. 2014. Invest Ophthalmol Vis Sci. 55:4295. PubMed
  7. Kumar V, et al. 2010. Blood. 115:4725. PubMed
  8. Carr T 2010. J Exp Med. 207:101. PubMed
  9. Jayaraman P, et al. 2012. J Immunol. 188:5365. PubMed
  10. Lu R, et al. 2012. Cancer Res. 72:2239. PubMed
  11. Kather J, et al. 2014. Invest Ophthalmol Vis Sci . 55:4295. PubMed
  12. Klose R, et al. 2016. Nat Commun. 7:12528. PubMed
  13. Bacigaluppi M, et al. 2016. J Neurosci. 36: 10529 - 10544. PubMed
  14. Nagao M,et al. 2017. Sci Rep.. 10.1038/s41598-017-13417-w. PubMed
Publication Library
RRID
AB_1027727 (BioLegend Cat. No. 512807)
AB_2212505 (BioLegend Cat. No. 512808)

Antigen Details

Structure
cystine-knot growth factors
Distribution

aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells

Function
angiogenesis
Cell Targets
endothelial cells
Receptors
VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1)
Antigen References

1. Contreras A, et al. 2007. Am. J. Transpl. 7:155.
2. Lambrechts D and Carmeliet P. 2007. Biochim. Biophys. Acta. 1762:1109.
3. Tong JP and Yao YF. 2006. Clin. Biochem. 39:267.
4. Ferrara N, et al. 2003. Nat. Med. 9:669.
5. Shibuya M. 2001. Cell Struct. Funct. 26:25.
6. Senger DR, et al. 1983. Science. 219:983.

Regulation
HIF-1, hypoxiaresponsiveness
Gene ID
22339 View all products for this Gene ID
UniProt
View information about VEGF-A on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house unless otherwise indicated. However upon request these can be tested from outside independent laboratories on custom basis.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 5    Revision Date: 10/17/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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