LEAF™ Purified anti-mouse IL-9 Antibody

Pricing & Availability
Clone
D9302C12 (See other available formats)
Other Names
Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype
Armenian Hamster IgG
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
504802 500 µg $300
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Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones. The D9302C12 antibody reacts with mouse IL-9. The D9302C12 antibody can neutralize the bioactivity of natural or recombinant mouse IL-9.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Baculovirus-expressed, recombinant mouse IL-9
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut, WB

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For neutralization of one unit/ml of IL-9, a concentration of 1-10 ug/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection1,2: The biotinylated D9302C12 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with Purified D8402E8 antibody (Cat. No. 504702) as the capture antibody.
Neutralization2-5:The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IL-9 bioactivity (Cat. No. 504802).

Additional reported applications (for the relevant formats) include: Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  2. Renauld, J., et al. 1995. J. Leuk. Biol. 57:353.
  3. Sitkauskiene, B., et al. 2005. Respir Res. 6:33.
  4. Jones, T.G, et al. 2009. J Immunol. 183:5251-60. (Neut) PubMed
  5. Jones, T.G, et al. 2010. J. Immunol. 185:1804-11. (Neut) PubMed
Product Citations
  1. Saeki M, et al. 2016. Allergol Int. doi: 10.1016/j.alit.2016.05.003. PubMed
  2. Do Thi VA, et al. 2018. Immune Netw. 18:e12. PubMed
Publication Library
RRID
AB_315346 (BioLegend Cat. No. 504802)

Antigen Details

Structure
Cytokine; 30-40 kD (Mammalian)
Bioactivity
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources
IL-2 activated TH2 lymphocytes
Cell Targets
T lymphocytes, erythroid precursors
Receptors
IL-9R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux, V. 1992. Research Immunology 143:385.
3. Renauld, J., et al. 1993. Adv. Immunol. 54:79.
4. Yang, Y. 1992. Leuk. Lymphoma 8:441.

Regulation
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID
16198 View all products for this Gene ID
UniProt
View information about IL-9 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 10/07/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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