- TC11-8H4 (See other available formats)
- Other Names
- Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
- Rat IgG1, κ
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- Product Citations
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IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. Recent study has shown that IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course. The TC11-8H4 antibody antibody reacts with mouse interleukin-17 (IL-17).Product Details
- Antibody Type
- Host Species
- E. coli-expressed, recombinant mouse IL-17A
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
ELISA Detection - Quality tested
- Application Notes
ELISA2 and ELISPOT3 Detection: The biotinylated TC11-8H4 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with Purified TC11-18H10 antibody (Cat. No. 506901) as the capture antibody, and with recombinant mouse IL-17 (Cat. No. 564101) as the standard.
(PubMed link indicates BioLegend citation)
- Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
- Herrada A, et al. 2010. J. Immunol. 184:191
- Nekrasova T, et al. 2005. J. Immunol. 175:2734.
AB_893543 (BioLegend Cat. No. 507004)
- Cytokine; dimer; 15 kD (Mammalian)
- Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
- Cell Sources
- CD4+ memory T cells
- Cell Targets
- Fibroblasts, epithelial and endothelial cells, stromal cells
- IL-17R (CD217)
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006 Nat. Rev. Immunol. 6(4):329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237(2):123.
- Gene ID
- 16171 View all products for this Gene ID
- View information about IL-17A on UniProt.org
- Does BioLegend test each LEAF™ antibody by functional assay?
No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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