- 20/70 (See other available formats)
- Other Names
- C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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CD88 is a single chain protein with seven membrane-spanning regions and a molecular weight of 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release, and superoxide anion production.Product Details
- Antibody Type
- Host Species
- mC5aR transfected cells
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
Block -Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications for the relevant formats include: blocking of C5a binding.
(PubMed link indicates BioLegend citation)
- Godau J, et al. 2004. J. Immunol. 173:3437.
- Shushakova N, et al. 2002. J. Clin. Invest. 110:1823.
- Kim SH, et al. 2013. Int Immunol. 25:623. PubMed
- Product Citations
AB_2067286 (BioLegend Cat. No. 135804)
- Single chain protein with seven membrane-spanning regions, 43 kD.
- Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.
- After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
- CD88 is coupled to heterotrimeric G proteins such as Gi, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
- Chemotaxis, degranulation.
- Cell Type
- Neutrophils, Eosinophils, Monocytes, Endothelial cells, Dendritic cells
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363.
3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429.
4. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719.
- Gene ID
- 12273 View all products for this Gene ID
- View information about CD88 on UniProt.org
- Does BioLegend test each LEAF™ antibody by functional assay?
No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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