- HM40-3 (See other available formats)
- Other Names
- Bp50, TNFRSF5
- Armenian Hamster IgM
- Ave. Rating
- 2 reviews
- Product Citations
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CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- (BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding1, stimulation of mouse B cell and dendritic cell proliferation2-6,9, and immunohistochemistry7 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102908).
(PubMed link indicates BioLegend citation)
- Kawano T, et al. 1997. Science 278:1626. (Block)
- Kaneko Y, et al. 1996. Eur. J. Immunol. 26:3061. (Costim)
- Kashiwada M, et al. 1996. Eur. J. Immunol. 26:1451. (Costim)
- Inaba M, et al. 1995. Eur. J. Immunol. 25:1244. (Costim)
- Akiba H, et al. 1999. J. Immunol. 162:7058. (Costim)
- Ridge JP, et al. 1998. Nature 393:474. (Costim)
- Morelli AE, et al. 2000. Transplantation 69:2647. (IHC)
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Yu X, et al. 2006. J. Biol. Chem. 281:15505. (Costim)
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Product Citations
AB_312950 (BioLegend Cat. No. 102907)
AB_312951 (BioLegend Cat. No. 102908)
- TNFR superfamily, 48 kD
B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors
- Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
- Ligand Receptor
- CD40L (CD154)
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
- Gene ID
- 21939 View all products for this Gene ID
- View information about CD40 on UniProt.org
- Does BioLegend test each LEAF™ antibody by functional assay?
No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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