LEAF™ Purified anti-mouse CD28 Antibody

Pricing & Availability
Clone
37.51 (See other available formats)
Other Names
Tp44, T44
Isotype
Syrian Hamster IgG
Ave. Rating
4 reviews
Product Citations
publications
37pt51_LEAF_081908.jpg
C57BL/6 splenocytes stained with LEAF™ purified 37.51, followed by anti-Syrian hamster IgG FITC
  • 37pt51_LEAF_081908.jpg
    C57BL/6 splenocytes stained with LEAF™ purified 37.51, followed by anti-Syrian hamster IgG FITC
Cat # Size Price Quantity Avail. Save
102111 50 µg $70
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102112 500 µg $230
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Description

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The CD28 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, IHC, Costim, Block - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.


This product is included in our Activation Bundles. Learn more...
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102112). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102116) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
  2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
  3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
  4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
  5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
  6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
  8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
  9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
  10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  11. Barbi J, et al. 2007. Blood 110:2215.
  12. Chen H, et al. 2009. Prostaglandins Leukot Essent Fatty Acids. 80:195. PubMed
  13. Milpied P, et al. 2011. Blood 118:2993. PubMed
  14. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
  15. Rybakin V, et al. 2012. PLoS One. 7:e43191. PubMed
  16. Myers RC, et al. 2013. J Immunol. 190:6287. PubMed
Product Citations
  1. Rybakin V, Gascoigne N 2012. PLoS One. 7:e43191. PubMed
  2. Myers R, et al. 2013. J Immunol. 190:6287. PubMed
  3. Chen H, et al. 2009. Prostaglandins Leukot Essent Fatty Acids. 80:195. PubMed
  4. Kimmerling R, et al. 2016. Nat Commun. 7:10220. PubMed
  5. Du C, et al. 2016. Nat Commun. 7: 11120. PubMed
  6. Nagashima H, et al. 2016. J Immunol. 196: 4082 - 4089. PubMed
  7. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  8. Hawila E, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.104. PubMed
  9. Moore MJ 2018. eLife. 7: e33057. PubMed
  10. Coley WD 2018. F1000Research. 7:318. PubMed
  11. Torgovnick A 2018. Cell reports. 25:1027. PubMed
  12. Smith LK 2018. Immunity. 48:299. PubMed
  13. Fan MY 2018. Cell reports. 25:1204. PubMed
  14. Sade-Feldman M 2018. Cell. 175:998. PubMed
  15. Tsai S 2018. Cell metabolism. 28:922. PubMed
Publication Library
RRID
AB_312876 (BioLegend Cat. No. 102111)
AB_312877 (BioLegend Cat. No. 102112)

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
Function
Costimulates T and NK cells
Ligand Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
Thymocytes, T cells, NK cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.

Gene ID
12487 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house unless otherwise indicated. However upon request these can be tested from outside independent laboratories on custom basis.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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