- 37.51 (See other available formats)
- Other Names
- Tp44, T44
- Syrian Hamster IgG
- Ave. Rating
- 4 reviews
- Product Citations
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CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.Product Details
- Antibody Type
- Host Species
- Syrian Hamster
- C57BL/6 mouse T-cell lymphoma EL-4
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
- The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The CD28 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
IP, IHC-F, Costim, Block - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
This product is included in our Activation Bundles. Learn more...
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102112). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102116) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
(PubMed link indicates BioLegend citation)
- Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
- Cibotti R, et al. 1997. Immunity 6:245. (Costim)
- Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
- Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
- Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
- Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
- Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
- Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
- Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
- Perchonock CE, et al. 2007. J. Immunol. 179:1768.
- Barbi J, et al. 2007. Blood 110:2215.
- Chen H, et al. 2009. Prostaglandins Leukot Essent Fatty Acids. 80:195. PubMed
- Milpied P, et al. 2011. Blood 118:2993. PubMed
- Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
- Rybakin V, et al. 2012. PLoS One. 7:e43191. PubMed
- Myers RC, et al. 2013. J Immunol. 190:6287. PubMed
- Product Citations
AB_312876 (BioLegend Cat. No. 102111)
AB_312877 (BioLegend Cat. No. 102112)
- Ig superfamily, 44 kD
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
- Costimulates T and NK cells
- Ligand Receptor
- CD80 (B7-1), CD86 (B7-2)
- Cell Type
- NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.
- Gene ID
- 12487 View all products for this Gene ID
- View information about CD28 on UniProt.org
- Does BioLegend test each LEAF™ antibody by functional assay?
No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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