LEAF™ Purified anti-mouse CD180 (RP105) Antibody

Pricing & Availability
Clone
RP/14 (See other available formats)
Other Names
CD180, Radio-protective 105-kD protein, RP105
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RPslash14_LEAF_090507
C57BL/6 mouse splenocytes stained with LEAF™ purified RP/14, followed by anti-rat IgG FITC
  • RPslash14_LEAF_090507
    C57BL/6 mouse splenocytes stained with LEAF™ purified RP/14, followed by anti-rat IgG FITC
Cat # Size Price Quantity Avail. Save
117708 500 µg $380
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Description

CD180 is a 105 kD type I membrane protein, also known as RP105 or Ly64. It is a member of the toll-like receptor family consisting of an extracellular leucine-rich repeat (LRR) and a short cytoplasmic tail. It is expressed on most B cells, monocytes/macrophages, and dendritic cells. CD180 associates with MD-1 to form the RP105/MD-1 surface receptor. CD180/MD-1, by working in concert with TLR4, controls B cell recognition and signalling of bacterial LPS. The ligation of RP105 by RP/14 leads to a strong B cell proliferative response, as well as protection from apoptosis induced by radiation or dexamethasone. Interestingly, in some systems, ligation of RP105 leads to Fas-independent apoptosis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse splenocytes
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, Activ, InductionProtection of Apop - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, B cell activation1,4,5, protection of B cells from apoptosis induced by radiation or dexamethasone and induction of Fas-independent apoptosis1,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 117708).

Application References

(PubMed link indicates BioLegend citation)
  1. Miyake K, et al. 1994. J. Exp. Med. 180:1217. (FC IP Activ Apop)
  2. Miyake K, et al. 1995. J. Immunol. 154:3333. (IP)
  3. Miyake K, et al. 1998. J. Immunol. 161:1348. (IP)
  4. Chan VWF, et al. 1998. J. Exp. Med. 188:93. (Activ)
  5. Yamashita Y, et al. 1996. J. Exp. Med. 184:113. (Activ Apop)
  6. Fujita K, et al. 2012. Immunol Lett. 144:1. PubMed.
RRID
AB_2072826 (BioLegend Cat. No. 117708)

Antigen Details

Structure
Member of the toll-like receptor family, 105 kD
Distribution

B cells, monocytes/macrophages, and dendritic cells

Function
Signaling protein involved in innate immunity
Ligand Receptor
LPS
Cell Type
B cells, Monocytes, Macrophages, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Miyake K, et al. 1994. J. Exp. Med. 180 :1217.
2. Miyake K, et al. 1995. J. Immunol.. 154:3333.
3. Miyake K, et al. 1998. J. Immunol.. 161:1348.
4. Chan VWF, et al. 1998. J. Exp. Med. 188 :93.
5. Yamashita Y, et al. 1996. J. Exp. Med. 184:113.

Gene ID
17079 View all products for this Gene ID
UniProt
View information about CD180 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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