LEAF™ Purified anti-mouse CD120a (TNF R Type I/p55) Antibody

Pricing & Availability
Clone
55R-170 (See other available formats)
Other Names
TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
Isotype
Armenian Hamster IgG
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Product Citations
publications
55R-170
NIH/3T3 cell line stained with purified 55R-170, detected with biotinylated anti-Armenian hamster IgG and Sav-PE
  • 55R-170
    NIH/3T3 cell line stained with purified 55R-170, detected with biotinylated anti-Armenian hamster IgG and Sav-PE
Cat # Size Price Quantity Avail. Save
112902 500 µg $300
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Description

CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. The 55R-170 antibody has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli-expressed extracellular domain of the mouse TNFRI protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, Block - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry: For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112904) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
ELISA Detection1: The biotinylated 55R-170 antibody is useful as a detection antibody when paired with the Purified 55R-286 antibody (Cat. No. 113002) as the capture antibody.
Additional reported applications (for the relevant formats) include:immunoprecipitation1, and In vitro and In vivo receptor blocking1. The LEAF™Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 112902).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan, K. C., et al. 1995. J. Exp. Med. 181:607.
RRID
AB_313527 (BioLegend Cat. No. 112902)

Antigen Details

Structure
TNFR superfamily, 55 kD
Distribution
Variety of cell types at low levels
Function
Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand Receptor
TNF-α, LT-α (TNF-β)
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Aggarwal, B. B., et al. 1985. Nature 318 665.
2. Chan, F. K.M., et al. 2000. Science 288:2351.
3. Loetscher, H., et al. 1990. Cell 61:351.
4. Rothe, J., et al. 1993. Nature 364:798.

Gene ID
21937 View all products for this Gene ID
UniProt
View information about CD120a on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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