LEAF™ Purified anti-human CD29 Antibody

Pricing & Availability
Clone
TS2/16 (See other available formats)
Workshop
V A-S202
Other Names
Integrin β1 chain, VLA-β chain, gpIIa, ITGB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TS216_LEAF_062107
Human peripheral blood lymphocytes stained with LEAF™ purified TS2/16, followed by anti-mouse IgGs FITC
  • TS216_LEAF_062107
    Human peripheral blood lymphocytes stained with LEAF™ purified TS2/16, followed by anti-mouse IgGs FITC
  • zTS2slash16_IF_072512
    BT474 breast cancer cells were stained with anti-CD29 (clone TS2/16) followed by DyLight™ 649 Goat anti-mouse Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
Cat # Size Price Quantity Avail. Save
303009 50 µg $110
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303010 500 µg $325
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Description

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cattle (Bovine, Cow), Cynomolgus, Dog (Canine), (Horse reactivity), Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IF - Validated
IP, IHC, Activ - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin β14,7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
  3. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
  4. Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
  5. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
  6. Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
  7. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
  8. Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
  9. Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
  10. Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
  11. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
  12. Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
Product Citations
  1. Barbolina M, et al. 2010. Mol Cancer Res. 0.786805556. PubMed
  2. Miura Y 2018. eLife. 7: e36572. PubMed
Publication Library
RRID
AB_314325 (BioLegend Cat. No. 303009)
AB_314326 (BioLegend Cat. No. 303010)

Antigen Details

Structure
Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α16), also associates with CD51 (αV), and α7- α9, 130 kD
Distribution

Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells

Function
Cell-cell and cell-matrix interactions
Ligand/Receptor
VCAM-1, MAdCAM-1, ECM
Cell Type
Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Monocytes, Platelets, Mesenchymal Stem Cells, Embryonic Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.

Gene ID
3688 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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