LEAF™ Purified anti-human CD158d (KIR2DL4) Antibody

Pricing & Availability
Clone
mAb 33 (33) (See other available formats)
Other Names
KIR2DL4, 2DL4, p49, KIR103
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
33_158d_PE_1_063011
Human peripheral blood lymphocytes were cultured 16 hrs with IL-2, then stained with CD56 (clone HCD56) APC and CD158d (clone 33) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 33_158d_PE_1_063011
    Human peripheral blood lymphocytes were cultured 16 hrs with IL-2, then stained with CD56 (clone HCD56) APC and CD158d (clone 33) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 33_158d_PE_2_063011
Cat # Size Price Quantity Avail. Save
347003 50 µg $195
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Description

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs, with long cytoplasmic domains, contain ITIMs and possess inhibitory functions and others, with short cytoplasmic regions, lack ITIM and have activation functions. Fourteen polymorphic KIR genes have been reported in humans. KIR2DL4 (CD158d) is a unique receptor which has an ITIM in its cytoplasmic domain and a charged residue in the transmembrane domain. It possesses both inhibitory and activation functions. Two common alleles (10A and 9A) of KIR2DL4 have been reported. The 10A allele (with 10 adenines at the end of the transmembrane exon) receptor is expressed on CD56high NK subset, whereas its expression on CD56dim NK cells is inducible upon culture. The major 9A allele receptor is a secreted form. HLA-G is the ligand of CD158d.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK3.3 cells and KIR2DL4-Ig fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Activ - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Intracellular staining is recommended to detect KIR2DL4 in NK cells, as this receptor resides predominantly in endosomes3. Alternatively, mAb 33 can be loaded into NK cells by endocytosis at 37°C3. This antibody also induces NK cell cytotoxicity1,2. Based on in-house observations, we suggest incubating for 45 - 60 minutes at room temperature with clone mAb 33 to observe better CD158d staining.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. Rajagopalan S, et al. 2001. J. Immunol. 167:1877. (FC)
  2. Goodridge JP, et al. 2003. J. Immunol. 171:1768. (FC)
  3. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
Product Citations
  1. Page M, et al. 2014. J Immunol. 192:732. PubMed
  2. Ueshima C, et al. 2015. Cancer Immunol Res. 3: 871-880. PubMed
  3. Fu B, et al. 2017. Immunity. 47:1100. PubMed
Publication Library
RRID
AB_2028425 (BioLegend Cat. No. 347003)

Antigen Details

Structure
Contains two Ig-like extracellular domains, 45-50 kD
Distribution

On CD56high NK cells and cultured CD56dim NK cells

Ligand Receptor
HLA-G
Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
2. Goodridge JP, et al. 2007. Eur. J. Immunol. 37:199.

Gene ID
3805 View all products for this Gene ID
UniProt
View information about CD158d on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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