Keratin 14 Polyclonal Chicken Antibody, Purified

Pricing & Availability
Clone
Poly9060 (See other available formats)
Other Names
Keratin, type I cytoskeletal 14, CK-14, cytokeratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
Previously
Covance Catalog# SIG-3476
Isotype
Chicken IgY
Ave. Rating
1 reviews
Product Citations
publications
Cat # Size Price Quantity Avail. Save
906001 100 µl $374
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Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly9060 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 14. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Reactivity
Human
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 14 protein.
Formulation
Phosphate-buffered solution + 0.1% sodium azide.
Preparation
The antibody was purified by IgY affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

IHC

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:1,000 with a biotin based detection system such as biotinylated anti-chicken immunoglobulin and USA Ultra Streptavidin Detection (Cat. No. 929501).

Positive Control tissue: Human skin

Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieval-All 1 solution (Cat. No. 927901, 928001, 928101).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC).

Application References

(PubMed link indicates BioLegend citation)
  1. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  2. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
Product Citations
  1. Papafotiou G, et al. 2016. Nat Commun. 7:11914. PubMed
  2. Wuidart A, et al. 2016. Genes Dev. 30: 1261 - 1277. PubMed
  3. Tao L, et al. 2017. Nat Commun. 8:14431. PubMed
  4. Linehan JL 2018. Cell. 172:784. PubMed
Publication Library
RRID
AB_2565055 (BioLegend Cat. No. 906001)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3861 View all products for this Gene ID 3861 View all products for this Gene ID
UniProt
View information about Keratin 14 on UniProt.org

Related FAQs

Does BioLegend manufacture antibodies at GMP grade?

BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at: https://www.biolegend.com/QC.

Go To Top Version: 4    Revision Date: 05/11/2017

Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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