Keratin 1 Polyclonal Antibody, Purified

Pricing & Availability
Clone
Poly19052 (See other available formats)
Other Names
Keratin, type II cytoskeletal 1, CK-1, cytokeratin-1, 67 kD cytokeratin, hair alpha protein, type-II keratin Kb1, epidermolytic hyperkeratosis 1, krt1
Previously
Covance Catalog# PRB-149P
Isotype
Rabbit Polyclonal
Ave. Rating
Submit a Review
Product Citations
publications
Purified_Keratin_Antibody_IHC_021615
Immunohistochemistry with anti-K1 (Cat: 905201) showing strong cytoplasmic staining of the epidermis in normal human skin tissue.
  • Purified_Keratin_Antibody_IHC_021615
    Immunohistochemistry with anti-K1 (Cat: 905201) showing strong cytoplasmic staining of the epidermis in normal human skin tissue.
Cat # Size Price Quantity Avail. Save
905201 100 µl $374
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly19052 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 1. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Reactivity
Human, Rat, Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the human keratin 1 protein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by peptide affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
Application

IHC

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:500 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC).


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wu N, Rollin J, Masse I, Lamartine J, and Gidrol X. p63 Regulates Human Keratinocyte Proliferation via MYC-regulated Gene Network and Differentiation Commitment through Cell Adhesion-related Gene Network. J. Biol. Chem.,287: 5627-5638, Feb 2012. PubMed
  2. Schütz M, Auth T, Gehrt A, Bosen F, K÷rber I, Strenzke N, Moser T, and Willecke K. The connexin26 S17F mouse mutant represents a model for the human hereditary keratitisùichthyosisùdeafness syndrome. Hum. Mol. Genet.; 20: 28-39, Jan 2011.
  3. Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
  4. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  5. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
Product Citations
  1. Wu N, et al. 2012. J Biol Chem. 287:5627-5638. PubMed
  2. Shigehara Y, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw277. PubMed
  3. Tanis SEJ 2018. Cell reports. 25:1292. PubMed
Publication Library
RRID
AB_2565047 (BioLegend Cat. No. 905201)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3848 View all products for this Gene ID 3848 View all products for this Gene ID
UniProt
View information about Keratin 1 on UniProt.org

Related FAQs

Does BioLegend manufacture antibodies at GMP grade?

BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at: https://www.biolegend.com/QC.

Go To Top Version: 4    Revision Date: 05/11/2017

Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account