Intracellular Staining Permeabilization Wash Buffer (10X)

Pricing & Availability
Other Names
Perm/Wash, Permeabilization Buffer
Ave. Rating
2 reviews
Product Citations
publications
Cat # Size Price Quantity Avail. Save
421002 100 ml $80
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Intracellular Staining Permeabilization Wash Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Intracellular Staining Permeabilization Wash Buffer is used to permeabilize cells following fixation with Intracellular Staining Fixation Buffer (Cat. No. 420801). It is supplied as a 10X solution and should be diluted in deionized water prior to use. Intracellular Staining Permeabilization Wash Buffer has been formulated to have minimal effects on cells, reduce non-specific staining and enhance the signal to noise ratio. It can be used for antibody dilutions and cell washing during intracellular staining.

Product Details
Technical data sheet

Product Details

Storage & Handling
The Intracellular Staining Perm wash buffer solution should be stored between 2°C and 8°C. Do not freeze.
Application

ICC, ICFC - Quality tested
IHC - Reported in the literature

Recommended Usage

For use in permeabilization, dilute Intracellular Staining Permeabilization Wash Buffer (10X) to 1X in DI water. Resuspend fixed cells in diluted Intracellular Staining Permeabilization Wash Buffer and centrifuge at 350 xg for 5-10 minutes, and repeat the process twice. It is recommended that the reagent be titrated for optimal performance for each application. Please see "Intracellular Cytokine Staining Protocol" on BioLegend's website for more details.

Application Notes

The Intracellular Staining Permeabilization Wash Buffer (10X) may have crystallization or precipitation observed when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is heavy precipitation observed after dilution to 1X working solution, the buffer can be filtered to clarify the solution.

Application References

(PubMed link indicates BioLegend citation)
  1. Smeltz RB. 2007. J. Immunol. 178:4786.
  2. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  3. del Rio ML, et al. 2011. Transplantation. 92:1085. PubMed
  4. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  5. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  6. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  7. Kusner LL, et al. 2014. PLoS One. 9:102231. PubMed
  8. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
Product Citations
  1. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  2. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  3. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  4. Xiao Z, et al. 2013. Mol Immunol. 56:423. PubMed
  5. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  6. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  7. Glaser K, et al. 2015. Immunol Lett. 166: 19-27. PubMed
  8. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  9. Pearce V, et al. 2015. J Immunol. 195: 3206 - 3217. PubMed
  10. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  11. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  12. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  13. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  14. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  15. Assadi G, et al. 2016. PLoS One. 11:e0168276. PubMed
  16. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  17. Nausch N, et al. 2017. PLoS Negl Trop Dis. 11(2):e0005415. PubMed
  18. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  19. Baek J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.011. PubMed
  20. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  21. Sun X, et al. 2017. Microbes Infect. 10.1016/j.micinf.2017.08.007. PubMed
  22. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  23. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  24. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  25. Goyal S, et al. 2017. Sci Rep. . 10.1038/s41598-017-18046-x. PubMed
Publication Library

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/26/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account