- Regulatory Status
- Other Names
- IL-17A ELISA Kit, IL-17 ELISA Kit
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- Product Citations
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IL-17 or IL-17A originally identified as cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) in mouse, is a founding member of the IL-17 family of cytokines. IL-17A is mainly produced by a small subset of T helper cells called Th17 cells. IL-17A is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with a molecular mass of 35 kD. The amino acid sequence of human IL-17A is 63% and 58% identical to mouse and rat IL-17A, respectively, and 72% identical to the ORF13 of Herpesvirus saimiri. IL-17A exhibits multiple biological activities including the induction of various proinflammatory cytokines and chemokines such as IL-6, IL-8, G-CSF, GM-CSF, GRO-α, and MCP-1 on a variety of cell types. IL-17A is also involved in the activation of NF-kB and costimulation of T cell proliferation. As a result of these roles, IL-17A has been linked to many immune/autoimmune related diseases including rheumatoid arthritis, asthma, lupus, and multiple sclerosis.
ELISA MAX™ Set Deluxe is specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
- Kit Contents
- Human IL-17A ELISA MAX™ Capture Antibody (200X)
- Human IL-17A ELISA MAX™ Detection Antibody (200X)
- Human IL-17A Standard
- Avidin-HRP (1000X)
- Substrate Solution A
- Substrate Solution B
- Coating Buffer A (5X)
- Assay Diluent A (5X)
- Verified Reactivity
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Product Citations
- 2 pg/mL
- Standard Range
- 3.9-250 pg/mL
- Materials Not Included
- Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
- Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
- Wash bottle or automated microplate washer
- Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Sealer
- Absorbent paper
- Do the ELISA MAX™ Deluxe Sets come with plates?
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.