ELISA MAX™ Deluxe Set Human FGF-basic

Pricing & Availability
Other Names
Fibroblast Growth Factor basic, FGF-2 (FGF2), bFGF, Heparin-binding growth factor 2 (HBGF-2, HBGF2), FGF b, FGFb, FGFbasic, FGF basic ELISA Kit
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Product Citations
publications
basic_Human_ELISA_Kit_Deluxe_042015
  • basic_Human_ELISA_Kit_Deluxe_042015
Cat # Size Price Quantity Save
434311 5 plates $315
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Description

The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number, but will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Fibroblast growth factor-basic (FGF-basic) is a heparin-binding growth factor which belongs to a family of Fibroblast Growth Factors (FGFs). FGFs are involved in many physiological and pathological processes including embryonic development, neuronal outgrowth, angiogenesis, and malignant transformation. FGF-basic is essential for the development and maintenance of vascular integrity during embryogenesis.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

The ELISA Max™ Set Deluxe is specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human FGF-basic ELISA MAX™ Capture Antibody (200X)
  • Human FGF-basic ELISA MAX™ Detection Antibody (200X)
  • Human FGF-basic Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer B (5X)
  • Assay Diluent A (5X)
  • Matrix Diluent A (for serum and plasma samples)

Product Details

Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
7.8 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized water
  • Phosphate-Buffered Saline: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) BioLegend Cat. No. 423001 is recommended.
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended.
  • Absorbent paper

Antigen Details

Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
2247 View all products for this Gene ID
UniProt
View information about FGF-basic on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe sets no longer come with plates, but Coating Buffer and Assay Diluent (for blocking and dilutions) are included in the ELISA MAX™ Deluxe Sets. Plates can be ordered separately (Cat. No. 423501), and instructions on coating the plates are in the sets’ product manuals.

Go To Top Version: 2    Revision Date: 04/03/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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