- Other Names
- Streptavidin-Horseradish Peroxidase, SAv-HRP
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- Product Citations
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Streptavidin binds to biotin with high affinity. Streptavidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA, ELISPOT, IHC or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For ELISPOT or Western blotting applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.Product Details
- Mouse, Human, Rat
- Buffered solution containing bovine protein and preservatives (methylisothiazolone, bromonitrodioxane, and other active isothiazolones).
- Streptavidin is conjugated with horseradish peroxidase under optimal conditions.
- Storage & Handling
This product is guaranteed for six months. The streptavidin-HRP solution should be stored undiluted between 2°C and 8°C. Avoid exposure to sodium azide.
This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
ELISA - Quality tested
ELISPOT, WB, IHC - Reported in the literature
- Recommended Usage
Each lot of this Streptavidin-HRP is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:3000 to determine optimal conditions. For ELISPOT analysis, the reagent should be titrated between 1:500 - 1:1500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Streptavidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Sav-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.
(PubMed link indicates BioLegend citation)
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- Product Citations
- Gene ID
- View information about Streptavidin on UniProt.org
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