HRP anti-TFAM Antibody

Pricing & Availability
Clone
18G102B2E11 (See other available formats)
Other Names
Transcription factor A, mitochondrial, mtTFA, MtTF1, TCF6, TCF-6, Transcription Factor 6
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_18G102B2E11_HRP_TFAM_Antibody_WB_050718.jpg
Western blot of HRP anti-TFAM antibody (clone 18G102B2E11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4 °C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 1_18G102B2E11_HRP_TFAM_Antibody_WB_050718.jpg
    Western blot of HRP anti-TFAM antibody (clone 18G102B2E11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4 °C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 2_18G102B2E11_HRP_TFAM_Antibody_IHC_050718.jpg
    IHC staining of HRP anti-TFAM antibody (clone 18G102B2E11) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4 °C. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
850503 25 µg $145
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850504 100 µg $315
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Description

TFAM (Transcription Factor A, Mitochondrial) binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation, and DNA replication and repair. The protein is required for accurate and efficient promoter recognition by mitochondrial RNA polymerase. Sequence polymorphisms in the TFAM gene are associated with Alzheimer's and Parkinson's diseases. Other diseases associated with TFAM include Mitochondrial DNA Depletion Syndrome, Hepatocerebral Form and Deoxyguanosine Kinase Deficiency.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human TFAM protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes endogenous TFAM in human brain tissues in WB and IHC applications but does not recognize mouse TFAM.

Application References

(PubMed link indicates BioLegend citation)
  1. Wang YE, et al. 2013. PLoS One 8(8):e74513. (WB)
  2. Caielli S, et al. 2016. J Exp Med. 213(5):697-713. (WB)
RRID
AB_2734628 (BioLegend Cat. No. 850503)
AB_2734629 (BioLegend Cat. No. 850504)

Antigen Details

Structure
TFAM is synthesized as a precursor form with a mitochondrial targeting peptide, which is removed by processing after import to the mitochondria. Mature TFAM has an apparent molecular weight of ~25 kD. Alternative splicing results in multiple transcript variants.
Distribution

Tissue distribution: Central nervous system, skin, kidney, lung, thyroid, lymph nodes, breast, esophagus, and heart.

Cellular distribution: Mitochondrion, nucleus, and cytosol.

Function
TFAM functions in mitochondrial transcription regulation, and DNA replication and repair.
Interaction
TFB1M, TFB2M, CLPX
Biology Area
Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Mitochondrial Markers
Antigen References

1. Bestwick ML, Shadel GS. 2013. Trends Biochem Sci 38(6):283-91.
2. Ramachandran A, et al. 2017. Nucleic Acids Res 45(2):861-874.
3. Günther C, et al. 2004. Neurosci. Lett. 369: 219–223.

Gene ID
7019 View all products for this Gene ID
UniProt
View information about TFAM on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/08/2018

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