HRP anti-Tau, 359-373 Antibody anti-Tau

Pricing & Availability

Clone: A16097B (See other available formats)
Regulatory Status: RUO
Other Names: Microtubule associated protein tau
Isotype: Rat IgG2b, κ
Ave. Rating: Submit a Review
Product Citations: publications

A16097B_HRP_Tau_359-373_Antibody_062818 — Western blot of HRP anti-Tau, 359-373 antibody (clone A16097B). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.20 µg/mL of the primary antibody overnight at 4 °C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.

Compare all formats

Cat #

Size

Price

Quantity Check Availability

Save

851404

100 µg

$311

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyperphosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human 4R Tau protein (K18) encompassing the microtubule binding domains.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody cross-reacts with 3R and 4R human Tau isoforms, and recognizes endogenous human, mouse, and rat Tau in brain lysates by WB.
RRID: AB_2734641 (BioLegend Cat. No. 851404)

Antigen Details

Structure

Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.

Distribution

Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.

Function

Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Interaction

Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

Tau

Antigen References

Augustinack JC, et al. 2002. Acta Neuropathol. 103(1):26-35. PubMed.
Seubert P, et al. 1995. J Biol Chem. 270(32):18917-22.PubMed.
Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.

Gene ID

4137 View all products for this Gene ID

UniProt

View information about Tau 359-373 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Western Blotting Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All Tau, 359-373 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Tau, 359-373	A16097B	WB,Direct ELISA,IHC-P
HRP anti-Tau, 359-373	A16097B	WB
Biotin anti-Tau, 359-373	A16097B	WB,IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/29/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.