HRP anti-Synaptotagmin-3 Antibody

Pricing & Availability
Clone
N278/19 (See other available formats)
Other Names
SYT3
Isotype
Mouse IgG1, κ
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Product Citations
publications
N278slash19_HRP_Synaptotagmin-3_Antibody_082718
Western blot of HRP anti-Synaptotagmin-3 antibody (clone N278/19). Lane 1: Molecular weight marker; Lane 2: 20 μg of rat brain lysate; Lane 3: 20 μg of mouse brain lysate. The blots were incubated with 0.1 μg/mL of clone N278/19 at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • N278slash19_HRP_Synaptotagmin-3_Antibody_082718
    Western blot of HRP anti-Synaptotagmin-3 antibody (clone N278/19). Lane 1: Molecular weight marker; Lane 2: 20 μg of rat brain lysate; Lane 3: 20 μg of mouse brain lysate. The blots were incubated with 0.1 μg/mL of clone N278/19 at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Cat # Size Price Quantity Avail. Save
852603 25 µg $100
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852604 100 µg $170
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Description

Synaptotagmin-3 (SYT3)  is a member of the synaptotagmin family, which consists of 13  membrane trafficking proteins that contain a N-terminal transmembrane region, and two cytoplasmic C2 domains. Synaptotagmin-3 is mainly localized at the presynaptic terminals in the central nervous system, where it functions as a Ca2+ sensor and plays an important role in exocytosis of secretory vesicles and neurotransmitter release. It has been shown that Ca2+ influx triggers the interaction between the C2-domains of SYT3 and the phospholipid membranes.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 442-533 (cytoplasmic C2B domain) of mouse Synaptotagmin-3
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Not recommended to be used in WB with human brain lysate.

RRID
AB_2750416 (BioLegend Cat. No. 852603)
AB_2750417 (BioLegend Cat. No. 852604)

Antigen Details

Structure
Synaptotagmine-3 is a 590 amino acid protein with a molecular mass of ~ 63 kD.
Distribution

Tissue Distribution: Central Nervous System
Cellular Distribution: Endosome, plasma membrane, synaptic vesicles, and cytosol.

Function
SYT3 functions as a Ca2+ sensor at the presynaptic terminals and plays a role in Ca2+-dependent exocytosis of secretory vesicles.
Interaction
SYT3 forms homodimers and heterodimers with SYT6, SYT9 and SYT10. It binds to SNARE-complex component syntaxin-1.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References
  1. Saheki Y, et al. 2017. Adv Exp Med Biol. 997:83
  2. Wang YL, et al. 2017. Cell Mol Life Sci. 74(16):2917
Gene ID
84258 View all products for this Gene ID
UniProt
View information about Synaptotagmin-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/28/2018

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