HRP anti-Synaptotagmin-12 Antibody

Pricing & Availability
Clone
N277/7 (See other available formats)
Other Names
Synaptotagmin-12, sytXII, synaptotagmin 12, Syt12, Synaptotagmin XII, Synaptotagmin-related gene 1 protein, Srg1, Sytr1
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
N277-7_HRP_Synaptotagmin-12_Antibody_052518
Western blot of HRP anti-Synaptotagmin-12 antibody. Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 10 μg of mouse brain lysate; Lane 4: 10 μg of rat brain lysate. The blots were incubated with 5 μg/mL of clone N277/7 or mouse IgG1 overnight at 4°C. The blot that was incubated with mouse IgG1 was incubated with HRP‐labeled goat anti‐mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N277-7_HRP_Synaptotagmin-12_Antibody_052518
    Western blot of HRP anti-Synaptotagmin-12 antibody. Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 10 μg of mouse brain lysate; Lane 4: 10 μg of rat brain lysate. The blots were incubated with 5 μg/mL of clone N277/7 or mouse IgG1 overnight at 4°C. The blot that was incubated with mouse IgG1 was incubated with HRP‐labeled goat anti‐mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
820403 25 µg $100
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820404 100 µg $170
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Description

Synaptotagmin 12 (Syt12) is regulated by protein kinase A (PKA) and is unique from other synaptotagmins in that it does not bind to Ca2+. Syt12 interaction with Syt1 prevents SNARE complex binding. Expression of Syt12 induces spontaneous neurotransmitter release, providing a mechanism for spontaneous synaptic-vesicle exocytosis that is Ca2+-independent.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2734583 (BioLegend Cat. No. 820403)
AB_2734584 (BioLegend Cat. No. 820404)

Antigen Details

Structure
Expected MW: 47 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
171180 View all products for this Gene ID
UniProt
View information about Synaptotagmin-12 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/29/2018

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