HRP anti-PICK1 Antibody

Pricing & Availability
Clone
L20/8 (See other available formats)
Other Names
PRKCA-binding protein, protein interacting with C kinase 1, protein kinase C-alpha-binding protein, protein that interacts with C kinase 1, protein kinase C, alpha binding protein, PRKCABP
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
L20-8_HRP_PICK1_Antibody_052918
Western blot of HRP anti-PICK1 antibody (clone L20/8). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blots were incubated with 1 μg/mL of clone L20/8 or mouse IgG2a overnight at 4°C. The blot that was incubated with mouse IgG2a was incubated with HRP‐labeled goat anti‐mouse IgG (Cat. No. 405306). HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
  • L20-8_HRP_PICK1_Antibody_052918
    Western blot of HRP anti-PICK1 antibody (clone L20/8). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blots were incubated with 1 μg/mL of clone L20/8 or mouse IgG2a overnight at 4°C. The blot that was incubated with mouse IgG2a was incubated with HRP‐labeled goat anti‐mouse IgG (Cat. No. 405306). HRP anti-β-tubulin antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
830903 25 µg $100
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830904 100 µg $170
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Description

Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function. (Uniprot)

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 10-130 of rat PICK1 protein.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and IHC-P.
This antibody is less reactive in human tissue in IHC-P.

Application References

(PubMed link indicates BioLegend citation)
  1. Evers DM, et al. 2010. Nat Neurosci. 13:1199.
RRID
AB_2734595 (BioLegend Cat. No. 830903)
AB_2734596 (BioLegend Cat. No. 830904)

Antigen Details

Structure
Expected molecular weight: 45 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
84591 View all products for this Gene ID
UniProt
View information about PICK1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/29/2018

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