HRP anti-Notch 1 Antibody

Pricing & Availability
Clone
N253/32 (See other available formats)
Other Names
Neurogenic locus notch homolog protein 1, mT14, p300, Motch A, Notch gene homolog 1, major type A protein, transmembrane receptor Notch1, N1, Mis6, Tan1, lin-12, 9930111A19Rik
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
N253-32_HRP_Notch-1_Antibody_1_061818_png
Western blot of HRP anti-Notch 1 antibody (clone N253/32). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of clone N253/32 or mouse IgG1 overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • N253-32_HRP_Notch-1_Antibody_1_061818_png
    Western blot of HRP anti-Notch 1 antibody (clone N253/32). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of clone N253/32 or mouse IgG1 overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • N253-32_HRP_Notch-1_Antibody_2_061818
    IHC staining of HRP anti-Notch 1 antibody (clone N253/32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N253-32_HRP_Notch-1_Antibody_3_061818
    IHC staining of HRP anti-Notch1 antibody (clone N253/32) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
819103 25 µg $100
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819104 100 µg $170
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Description

Notch 1, also known as TAN-1, is a transmembrane protein. Its extracellular domain contains 29 epidermal growth factor-like (EGF) repeats and 3 Lin/Notch Glp (LNR) repeats, the intracellular domain contains 5 CDC10/Ankryn repeats (ANK), 1 proline, glutamate, serine, threonine-rich (PEST) motif, and 1 regulation of amino acid metabolism 23 (RAM23) domain. Notch 1 regulates the development, differentiation, and survival of a broad spectrum of cell lineages. It is involved in myogenesis, neurogenesis, gliogenesis, and lymphocyte development, resulting in Notch 1 expression in many organs such as brain, lung, thymus, spleen, bone marrow, spinal cord, eyes, mammary gland, liver, intestine, kidney, and heart. Notch 1 ligands are Jagged 1, Jagged 2, Delta 1, and Delta 4. Upon ligand binding, the intracellular domain of Notch 1 is cleaved and translocates to the cell nucleus where it forms a transcriptional activator complex with RBP-J κ.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is less reactive in human tissue in IHC-P.

RRID
AB_2734579 (BioLegend Cat. No. 819103)
AB_2734580 (BioLegend Cat. No. 819104)

Antigen Details

Structure
Expected molecular weight: >270 kD, 120 kD
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
18128 View all products for this Gene ID
UniProt
View information about Notch 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/20/2018

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